枳煿子乙酸乙酯提取物对大鼠肝微粒体细胞色素P450的影响  

Effects of the Ethyl Acetate Extract of Semen Hoveniae on Liver Microsomal Cytochrome P450 System in Rats

作　　者：张洪[1] 宋娟[1] 谭晔[1] 詹新安[1] 

机构地区：[1]武汉大学人民医院药学部,湖北武汉430072

出　　处：《中国实验方剂学杂志》2007年第3期37-39,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省科技攻关项目(202AA301C61)

摘　　要：目的:观察枳煿子乙酸乙酯提取物胶囊对大鼠肝微粒体细胞色素P450及其同工酶的影响。方法:大鼠分别灌服给予枳煿子乙酸乙酯提取物胶囊相当于生药量0.14 g/kg,0.17 g/kg和0.2 g/kg,连续10 d,用紫外分光光度法测定细胞色素P450及其同工酶的活性。结果:枳煿子乙酸乙酯提取物胶囊对细胞色素P450及NADPH-细胞色素C(P-450)还原酶含量无显著影响;可抑制苯胺羟化酶活性,抑制率可达31.5%;可增加氨基比林N-脱甲基酶活性,可达42.4%;而对红霉素N-脱甲基酶的活性没有明显影响。结论:枳煿子乙酸乙酯提取物胶囊对大鼠肝细胞色素P450、NADPH-细胞色素C(P-450)还原酶及红霉素N-脱甲基酶无明显作用,但对氨基比林N-脱甲基酶活性及苯胺羟化酶活性有显著影响。Objective：To investigate the effects of the ethyl acetate extract of Semen Hoveniae capsule on liver microsomal cytochrome P450 system in rats. Methods：The rats were given orally the extract in the doses of 0.14 g/kg, 0.17 g/kg and 0.2 g/kg（equivalent to the crude herb） for 10 days respectively. The activities of cytochrome P450 enzymes were measured by UV chromatography. Results： The cytochrome P450 content, NADPH-Cyt C reductase activities and erythromycin N-demethylase activities were not affected; Aniline hydroxylase activities in liver were decreased by up to 35.1% ;aminopyrine N-demethylase activities in liver were increased by up to 42.4%. Conclusions： The ethyl acetate extract of Semen Hoveniae capsule had no inhibition effect on the liver microsomal cytochromd P450, NADPH-Cyt C reductase and levels erythromycin N-demethylase in rats demethylase activities were significantly affected. However, analine hydroxylase activities and aminopyrine N- demethylase activities were significantly affected.

关 键 词：枳棋子 乙酸乙酯提取物 细胞色素P450 

分 类 号：R285.5[医药卫生—中药学]