奈替米星体液浓度测定的方法学研究  

Methodological study on assay of netilmicin concentration in body fluid

作　　者：汪冰[1] 张慧琳[1] 吕晓菊[1] 朱淑媛[1] 俞汝佳[1] 冯萍[1] 卢毅[1] 

机构地区：[1]华西医科大学附属第一医院

出　　处：《中国抗生素杂志》1997年第2期115-117,114,共4页Chinese Journal of Antibiotics

摘　　要：通过对国产硫酸奈替米星注射后人体血尿中药物浓度测定，建立了奈替米星体液浓度测定的微生物法，该方法的检测限为０．０３７５～０．０７５１μｇ，最低检测浓度为０．２５～０．５μｇ／ｍｌ，回收率为８８．８％±３．６３％～９８％±１４．４０％，重复性试验相对标准差为２．２２％～５．３４％；并对１０例健康受试者用药后血标本１００余份进行了微生物法测定和高压液相色谱法测定，对两种方法测定结果的比较发现：微生物法测定的血药浓度明显高于ＨＰＬＣ法（Ｐ＜０．０１），而ＨＰＬＣ法由于需复杂的样品预处理和特殊的色谱条件，测定的血药浓度较低，回收率仅７１．９８％～７６．９６％。不过，两种方法测定结果的相关性较好。本研究结果提示：微生物法测定奈替米星体液浓度简便、易操作、结果准确可信，适用于体液药物浓度测定。A microbiological method has been established for assay of domestic netilmicin concentration in body fluid. By using this method, the sensitivity, lowest concentration and reabsorption rate reached 0.0375￣0.0751μg, 0.25￣0.5μg/ml and 88.8%±3.63%￣98%±14.40% respectively. The relative standard difference (RSD) of repeated test was 2.22%￣5.34%. One hundred blood samples from 10 healthy volunteers have been assayed by this method and HPLC. The blood con centration determined by former method was much higher than that by the later one ( P < 0.01 ). The concentration lowered by HPLC was due to the need of complicated pretreatment of samples and specific chromatographic condition. The reabsorption rate of HPLC was only 71.98%￣76.96%. However, the correlation between the results of the two methods was satisfactory. Results suggested that microbiological method with the advantages of con venience, simplicity, high accuracy and reliability can be used to assay the netilmicin concentration in body fluid.

关 键 词：奈替米星 体液浓度 

分 类 号：R978.12[医药卫生—药品]