利用SRAP和SSR分子标记检测分析29份棉花种质遗传完整性  

Genetic Integrity Analysis of Cotton (Gossypium hirsutum Linn.) Accessions Using SRAP and SSR Markers

作　　者：李驰[1] 卢新雄[1] 张志娥[1] 陈晓玲[1] 林凤[2] 张香云[3] 刘存敬[3] 

机构地区：[1]中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室,北京100081 [2]沈阳农业大学,沈阳110016 [3]河北省农林科学院棉花研究所,石家庄050051

出　　处：《植物遗传资源学报》2007年第1期21-25,29,共6页Journal of Plant Genetic Resources

基　　金：国家"十五"攻关项目(2004BA525B01)

摘　　要：利用SRAP和SSR分子标记检测29份棉花种质遗传完整性。结果表明,无论每一份不同更新发芽率水平繁殖后代的种质之间,还是每一份不同繁殖世代数种质之间,其等位基因频率差异不显著,也没有检测到稀有等位基因缺失的情况。本试验表明不同更新发芽率水平和繁殖世代数差异没有对棉花这种常异花授粉作物种质遗传完整性变化产生影响。Genetic integrity of 29 cotton （Gossypium hirsutum Linn. ） accessions was analyzed using SRAP and SSR markers. It showed that the differences of allele frequency among the populations regenerated from the accession samples with different germination level or the populations obtained from the accession samples with different generations were not significant, and there was no absence of rare alleles observed. The result showed that the factors of different germination levels and generation times did not affect the genetic integrity during the regeneration in the case of often cross-pollinated crops like cotton.

关 键 词：棉花 SSR SRAP 遗传完整性 种质资源 保存 

分 类 号：S562[农业科学—作物学]