O型口蹄疫单克隆抗体6H4株金标试纸条的制备  

Preparation of colloidal gold labelling strips with the 6H4 strain of monoclonal antibody against the type O foot and mouth disease

作　　者：林彤[1] 常惠芸[1] 吴海波[1] 邵军军[1] 独军政[1] 丛国政[1] 李克生 杜惠芬 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室 [2]甘肃省医学科学院医学生物技术中心,甘肃兰州730050

出　　处：《甘肃农业大学学报》2007年第3期20-23,共4页Journal of Gansu Agricultural University

基　　金：国家"863"高技术研究发展计划(2003AA241110).

摘　　要：以制备的O型口蹄疫单克隆抗体为基础,结合免疫金技术,采用柠檬酸三钠还原法制备胶体金颗粒,对6H4单克隆抗体进行标记,制备了该单克隆抗体的金标试纸条.研究结果表明,6H4单克隆抗体在pH 8.64下的稳定点为12 mg/L,可制备成金标记抗体及其反应膜.Based on prepared monoclonal antibody（McAb） against the type O foot and mouth disease virus（FMDV） ,the 6H4 strain of McAb was applied to develop a new kind of McAb colloidal gold labelling strips by immune colloidal gold technique, aiming at obtaining a extraodinary, handy, rapid and audio-visual way of foot and mouth disease（FMD） detection and diagnosis. The colloidal gold particles were synthesized were prepared by sodium citrate reduction method and used as the labelling subjects of the 6H4 McAb. The results showed that the 6H4 strain of McAb was able to prepare the colloidal gold labelling antibody and its reactive membrane,and the concenration stable point of its McAb purified protein in the pH8. 64 colloidal gold solution was calculated to be 12 mg/L.

关 键 词：口蹄疫 单克隆抗体 金标试纸条 

分 类 号：S852.65[农业科学—基础兽医学]