SD大鼠气管黏膜下注射硬化剂后VEGF表达及成纤维细胞的增生  

Expression of VEGF and Hyperplasia of Fibroblast in SD Rats Trachea Submucosa after Injection with Polidocanol

作　　者：张春荣[1] 郑如恒[2] 葛棣[2] 

机构地区：[1]江苏省南通市肿瘤医院胸外科,江苏南通226361 [2]复旦大学附属中山医院胸外科,上海200032

出　　处：《中国临床医学》2007年第3期327-328,共2页Chinese Journal of Clinical Medicine

摘　　要：目的:研究SD大鼠气管黏膜下层注射硬化剂对血管内皮生长因子(VEGF)表达及成纤维细胞增生的影响。方法:将54只SD大鼠随机分成正常组(6只)、对照组(24只)和硬化剂组(24只),后两组按第1、3、5和7天4个时间点各6只。正常组不作处理,对照组仅用空针刺激气管黏膜下层,硬化剂组用1%乙氧硬化醇0.05 ml气管黏膜下注射,分别在第1、3、5和7天取标本作VEGF免疫组化染色,用Image Pro Plus 5.1图像分析软件进行半定量分析积分光密度.并在电镜下观察成纤维细胞的增生变化。结果:(1)硬化剂组在4个时间点的VEGF积分光密度均明显高于对照组(P<0.01)。在硬化剂组内,第3天明显高于第1天,第3和第5天无明显差异,而第5天又高于第7天。(2)电镜下硬化剂组成纤维细胞从第3天开始明显增多,并有胶原纤维形成,第5天达到高峰,胶原纤维变致密,并出现弹力纤维,至第7天成纤维细胞开始破碎、分解。结论:硬化剂能够明显促进SD大鼠气管黏膜下层VEGF的表达和成纤维细胞的增生,作用高峰时间在第3～5天。Objective：To study the expression of vascular endothelial growth factor（VEGF） in SD Rats＇ trachea submucosa and to observe the fibroblast with microscope. Methods： 54 SD Rats were randomly divided into normal group（n = 6） , empty needle injection group （n = 24） and polidocanol injection group（n = 24）. The latter two groups were equally divided into four subgroups according to the time of Ist day, 3^rd day, 5^th day and 7^th day. The Rats＇ trachea submucosa were injected with empty needle or 0.05ml 1% polidocanol, and the normal group without any interference. All the samples were stained by immunohis tochemistry, and were observed by electron microscopes. The integrated optical density（IOD） of VEGF were analyzed with Image Pro Plus 5.1 software. Results： （1） The IOD of VEGF in the polidocanol injection group was higher than those in the empty needle injection group （P〈0.01）. Within the polidocanol injection group, the IOD of VEGF was much higher in the 3^rd day than in the 1^st day, and it was also higher in the 5^th day than in the Th day, but there was no difference between the 3rd and 5^th day. （2） According to the electron microscope, the fibroblast in the polidocanol injection group were much more than those in the normal group. Collagen fiber bundles present from the 3^rd day and became denser and more tightly packed from the 5^th day to the Th day. Conclusion：Polidocanol can distinctly increase the expression of VEGF in SD Rats＇ submucosa and visibly promote the hyperplasia of fibroblast, and the apparently reaction time was in the 3^rd and 5^th day.

关 键 词：1%乙氧硬化醇 黏膜下层 VEGF 成纤维细胞 

分 类 号：R562[医药卫生—呼吸系统]