DNA Cleavage Promoted by Cu^2＋ Complex of N,N＇-Bis（2-aminoethyl）-2,6-pyridinedicarboxamide  

作　　者：李英 盛欣 邵莺 陆国元 

机构地区：[1]Department of Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, Jiangsu 210093, China

出　　处：《Chinese Journal of Chemistry》2007年第7期937-941,共5页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No. 20372032） and the Analytical Foundation of Nanjing University.

摘　　要：The interaction of Cu^2＋ complex of N,N＇-bis（2-aminoethyl）-2,6-pyridinedicarboxamide （BAP） with DNA was studied by agarose gel electrophoresis analysis. The results indicate that the BAP-Cu^2＋ complex can promote the cleavage of phosphodiester bond of supercoiled DNA at physiological condition, which is 3.2 × 10^6 times higher than DNA natural degradation. A hydrolytic cleaving mechanism through the cooperation of copper ions and functional amino groups was proposed.The interaction of Cu^2＋ complex of N,N＇-bis（2-aminoethyl）-2,6-pyridinedicarboxamide （BAP） with DNA was studied by agarose gel electrophoresis analysis. The results indicate that the BAP-Cu^2＋ complex can promote the cleavage of phosphodiester bond of supercoiled DNA at physiological condition, which is 3.2 × 10^6 times higher than DNA natural degradation. A hydrolytic cleaving mechanism through the cooperation of copper ions and functional amino groups was proposed.

关 键 词：DNA cleavage phosphodiester bond ELECTROPHORESIS nuclease model 

分 类 号：Q523[生物学—生物化学]