温度和pH值对重组杆状病毒(AcMNPV-BmKIT-Chi)的毒力影响  

Effect of Temperature and pH on Virulence of Recombinant Baculovirus (AcMNPV-BmKIT-Chi)

作　　者：范晓军[1] 许成钢[1] 柴宝峰[1] 王伟[1] 梁爱华[1] 

机构地区：[1]山西大学生物技术研究所/化学生物学与分子工程教育部重点实验室,太原030006

出　　处：《应用与环境生物学报》2007年第3期338-340,共3页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.39970416;30270204;30300038);山西省自然科学基金项目(No.031010)~~

摘　　要：研究了温度、酸碱度对重组杆状病毒（AcMNPV—BmKIT-Chi）毒力的影响．结果显示，多角体病毒在温度4～50℃，pH6．0～8．0范围内可以保持较高的毒力稳定性；不同虫龄棉铃虫幼虫对重组病毒的毒力敏感性差异显著，低虫龄对重组核型多角体病毒更为敏感；病毒对幼虫存在亚致死作用，饲毒组与对照组幼虫体重差异显著．Temperature and pH stability for recombinant baculovirus （AcMNPV-BmKIT-Chi） which expresses neurotoxin form scorpion and chitinase from Manduca secta was studied in this paper. The results indicated that the recombinant baculovirus kept relative high virulence stability under the condition of 4 ~ 50 ~C and pH 6.0 ~ 8.0. Larvae （Heliothis armigera） at different instars showed different sensitivities to the recombinant baculovirus. The second instar larvae were more sensitive to the recombinant baculovirus than the third instar, which were in turn more sensitive than the forth instar. Significant difference existed in weight between the infected group and the control group. Fig 3, Tab 3, Ref 13

关 键 词：温度 酸碱度 重组杆状病毒 毒力 稳定性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]