复方六月雪体外抗HBV的实验研究  

The inhibitory effect of compound Liuyuexue on on HBV in vitro

作　　者：杨霞芳[1] 张士军[2] 黄春喜[2] 黄仁彬[2] 

机构地区：[1]广西壮族自治区人民医院检验科 [2]广西医科大学药理学教研室,南宁530021

出　　处：《内科》2007年第3期317-319,共3页Internal Medicine

基　　金：广西科学研究与技术开发计划项目(桂科攻0322024-5E)

摘　　要：目的观察复方六月雪(CLYX)体外抗乙型肝炎病毒(HBV)的作用。方法在最大无毒浓度(TC0)基础上观察不同浓度药物作用于HepG2.2.15细胞,分别在第4天和8天收集细胞培养上清液,采用实时荧光定量PCR法检测上清液HBV DNA的含量,采用ELISA法测定上清液HBsAg和HBeAg的滴度。结果无毒浓度的复方六月雪在HepG2.2.15细胞培养中可有效地抑制细胞HBV DNA的复制及HBsAg和HBeAg的分泌。结论CLYX在体外有显著的抗HBV的作用。Objective To study the inhibitory effect of traditional Chinese medicine compound Liuyuexue on HBV in vitro. Methods At non-cellulotoxic concentration ,the different concentrations of CLYX were added respectively to HepG2.2.15 cells, which were incubated subsequently. 4 days and 8 days after treatment with drugs, the culture media were collected for determination of the quantity of HBV DNA detected by real time fluorescence quantitative PCR （FQ-PCR） and the levels of HBsAg and HBeAg by ELISA. Results CLYX at the largest non-cellulotoxic concentration could decrease the HBV DNA level and suppress HBsAg and HBeAg expression in HepG2.2.15 cell. Conclusion CLYX can inhibit significantly HBV in vitro.

关 键 词：复方六月雪 HepG2.2.15细胞培养 荧光定量PCR 抗乙型肝炎病毒 

分 类 号：R285[医药卫生—中药学]