新城疫病毒感染对小鼠肉瘤S180细胞p53蛋白的表达及细胞周期的影响  

Effects of Newcastle Disease Virus Infection on the Expression of p53 and Cell Cycle in Mouse Sarcoma S180 Cells

作　　者：程建贞[1] 孙黎[1] 翟登高[1] 薄爱华[2] 张进顺[1] 

机构地区：[1]河北北方学院医学技术学院免疫教研室,河北张家口075000 [2]河北北方学院实验中心,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2007年第3期3-8,共6页Journal of Hebei North University:Medical Edition

摘　　要：目的:研究新城疫病毒(NDV)感染对小鼠肉瘤S180细胞p53蛋白的表达及细胞周期的影响。方法:通过NDV体外感染小鼠肉瘤S180细胞,经倒置显微镜观察细胞形态变化,噻唑蓝(MTT)比色法检测小鼠肉瘤S180细胞的增殖状况;经流式细胞仪分析检测NDV体内感染后荷瘤鼠腹水中S180细胞分裂周期各时相的变化、细胞凋亡情况及细胞表面p53蛋白的表达情况。结果:NDV体内、外感染对小鼠肉瘤S180细胞的杀伤作用明显,NDV体内感染后荷瘤鼠腹水中S180细胞高表达p53蛋白,细胞凋亡率增加,G2/S期细胞减少,增殖指数(PI)降低,与阴性对照组比较有显著性差异(P<0.05)。结论:NDV感染可增强小鼠肉瘤S180细胞p53蛋白的表达,影响其细胞周期,诱导其凋亡且有较强的杀瘤作用。Objective. To investigate the effects of Newcastle Disease Virus infection in vitro and vivo on the expression of p53 and cell cycle in Mouse Sarcoma S180 cells. Methodss Atfter infection with NDV in vitro, study the alteration of morphology and proliferation of Mouse Sarcoma S180 cells by means of inverted microscope and MTT assay. The cell apoptosis, alteration of every phase of cell cycle, cell proliferation index （PI） and the expression of p53 of S180 cells infected by different dosage NDV were checked by fluorescence activated cell sorter（FACS）. Results.. The function of killing by NDV infection in vitro and vivo was shown. The over expression of p53 protein and induced apoptosis of S180 cells, reduced G2-phase and S-phase cells, meanwhile, proliferation index declined. Compared with negative control group, the effect of various stresses was significant（P〈0. 05）. Conclusion. Killer action of the NDV infection to S180 cells was obvious both in vitro and in vivo.

关 键 词：新城疫病毒 小鼠肉瘤S180细胞 P53蛋白 细胞周期 流式细胞术 

分 类 号：R373[医药卫生—病原生物学]