自身树突状细胞疫苗治疗尖锐湿疣的临床研究  

Auto-dendritic cell-vaccine in treatment of patients with condyloma acuminatum:a clinical study

作　　者：施晓琴[1] 刘军权[2] 陈复兴[2] 陈维峰[1] 

机构地区：[1]解放军第97医院皮肤科,江苏徐州221004 [2]解放军第97医院实验科,江苏徐州221004

出　　处：《中国肿瘤生物治疗杂志》2007年第3期275-278,共4页Chinese Journal of Cancer Biotherapy

基　　金：南京军区医学科研"十一五"计划资助项目(No06MA45)~~

摘　　要：目的:观察自身树突状细胞疫苗对人乳头状瘤病毒(human papillomavirus,HPV)感染所致尖锐湿疣患者T细胞的作用和临床治疗效果。方法:入选的73例受试者均为HPV-DNA检测阳性,并经过冷冻、或激光、或电外科治疗加干扰素抗病毒治疗半年未愈的尖锐湿疣(condyloma acuminatum,CA)患者。抽取患者外周血分离单个核细胞(PBMC),用细胞因子联合定向培养树突状细胞(DC);从CA患者的疣细胞中提取HPV抗原,用其致敏自身DC制备DC疫苗;将DC疫苗与自身T淋巴细胞共同培养后,检测T细胞增殖数;同时将自身DC疫苗注入患者腹股沟的浅表淋巴结内,每例注入DC总数为(1～4)×107个,观察其疗效。结果:经HPV致敏的DC疫苗能促进自身T淋巴细胞增殖,与对照组比较有显著性差异(P<0.01)。经HPV致敏DC疫苗治疗后,治愈率为90.4%(66/73),经6～12个月随访无复发;每例治愈时间为(25±10)d。结论:HPV致敏的自身DC疫苗能提高自身T淋巴细胞增殖能力,治疗HPV致尖锐湿疣疗效显著,无不良反应。Objective： To observe the effects of auto-dendritic cell vaccine on T cells of patients with condyloma acuminatum （CA） related human papilloma virus （HPV） and its clinical outcome. Methods： 73 patients with condyloma acuminata （CA） in the present study were positive of HPV-DNA and have been unsuccessfully treated with freezing, laser, or electric therapy combined with interferon anti-virus treatment for half a year. The peripheral blood samples were harvested from patients and the monouclear cells were extracted to cultivate DCs in presence of cytokines. Auto DCs were sensitized with corresponding HPV virus antigen of a patient to prepare the auto-dendritic cell vaccine. Then the vaccine were cultivated with auto T-cells and the proliferation of T cells were examined. Meanwhile, the auto-DC vaccine （1-4） × 10^7 was also injected into the patients＇ inguinal lymph glands and its therapeutic effectiveness was observed. Results： The prepared DC-vaccine significantly promoted the proliferation of auto T cells compared with control group （ P 〈 0.01 ）. Treatment with auto-DC vaccine had an effective rate of 90.4% （66/73） in the 73 patients, and there was no recurrence after 6-12 months＇ follow-up. The average time needed for therapy was （25 ± 10） d. Conclusion： The prepared auto-DC vaccine can boost auto T lymph cell proliferation and has obvious curative effect on HPV-related CA, with no apparent side effects.

关 键 词：树突状细胞疫苗 人类乳头状瘤病毒 尖锐湿疣 淋巴细胞增殖 

分 类 号：R752.5[医药卫生—皮肤病学与性病学]