油松种胚愈伤诱导的初探  

A Primary Study on the Callus Inducement from Embryo of Pinus tabulaeformis

作　　者：周巧云[1] 郑彩霞[1] 赵程亮 

机构地区：[1]北京林业大学生物科学与技术学院林木花卉遗传育种教育部重点实验室,北京100083 [2]银川建发集团,宁夏银川750001

出　　处：《江西农业大学学报》2007年第3期409-412,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30371144)

摘　　要：以油松成熟种子为材料,研究了外植体的不同处理方法、不同培养基、激素浓度及光照条件对愈伤诱导的影响。结果表明,在暗培养条件下,以带子叶的种子段为外植体,在DCR+NAA 1 mg/L+6-BA 0.2 mg/L培养基上可以获得诱导率较高的愈伤,诱导率为66%。Using the mature seeds of Pinus tabulaeformis as material, the effects of different methods on dealing with the explants,hormone concentration, media and illumination conditions on the callus inducement of Pinus tabulaeformis were studied. The results showed that in the dark environment, when the part of seed with the cotyledon was chosen as the explants callus,the inducement rate was higher in the medium of DCR ＋ NAA mg/L ＋ 6 - BA mg/L than in other media, and the callus inducement rate was 66%.

关 键 词：油松 激素 愈伤组织 诱导 

分 类 号：Q949.665[生物学—植物学]