RT-PCR检测猪粪便中猪繁殖与呼吸综合征病毒方法的建立  

Establishment of RT-PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus RNA from Pig Feces

作　　者：刘才根[1] 何后军[1] 罗咏梅[1] 邬向东[1] 潘金发[1] 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045

出　　处：《江西农业大学学报》2007年第3期434-436,442,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省科技厅资助项目(30160046);江西省教育厅资助项目(20051B0204900)

摘　　要：通过逆转录-聚合酶链扩增(RT-PCR)反应,建立从猪粪便中检测猪繁殖与呼吸综合征病毒(PRRSV)RNA的方法,以此方法检测了2个猪场8份样本,结果显示6份猪繁殖与呼吸综合征病毒阳性。In this experiment,the RT - PCR for detection of porcine reproductive and respiratory syndrome virus （PRRSV）RNA from pig feces has been established. 8 pig feces samples from 2 pig farms have been detected by this method,the results showed that 6 samples were PRRSV positive.

关 键 词：PRRSV 逆转录-聚合酶链式反应(RT-PCR) 建立 

分 类 号：S828[农业科学—畜牧学] S851.347.101[农业科学—畜牧兽医]