牛心朴子草中生物碱的抑制植物病毒活性研究(英文)  

Antiviral Activity of Alkaloids from Cynanchum komarovii

作　　者：黄治强[1] 刘玉秀[1] 范志金[1] 汪清民[1] 李广仁[1] 姚宇澄[2] 于学舜[2] 黄润秋[1] 

机构地区：[1]南开大学元素有机化学国家重点实验室,元素有机化学研究所,天津300071 [2]内蒙古工业大学化工学院,内蒙古呼和浩特010062

出　　处：《精细化工中间体》2007年第3期20-24,46,共6页Fine Chemical Intermediates

基　　金：国家重点基础研究发展规划项目(2003CB114404);国家自然科学基金资助项目(20472039,20421202);新世纪优秀人才支持计划资助项目(NCET-04-0228);教育部科学技术研究重点项目(106046)。

摘　　要：牛心朴子草广泛分布于我国西北旱沙荒漠地带,笔者发现其提取物中生物碱部位对危害极大的烟草花叶病毒(TMV)具有很高的抑制活性。生物活性跟踪与色谱分离、结构鉴定确认该生物碱属于菲并吲哚里西啶,活性成份为安托芬(1)和6-羟基-2,3-二甲氧基菲并吲哚里西啶(2)。此外,还分离得到另外三种生物碱。构效关系表明氮上孤电子对和菲环对高的抗病毒活性是必要的。With the aim of searching antiviral substances to tobacco mosaic virus （TMV）, alkaloid extracts from the alcohol extract of Cynanchum komarovii were isolated through gel chromatograph. 7-Demethoxytylophorine （antofine, 1） was present as the primary content of the alkaloid, another four alkaloids, 6 -hydroxyl -2,3 - dimethoxyphenanthroindolizidine （2-）, 7-demethoxytylophorine N-oxide （3）, 14-hydroxyantofine N-oxide （4） and 2,3 -dimethoxy-6-（3 -oxobutyl） -7,9,10,11,11a, 12-hexahydrobenzo [ f] pyrrolo [ 1,2-b] isoquinoline （5） were obtained at lower levels in the extract. Among them, the known alkaloid （2） was isolated from Cynanchum komarovii for the first time. A half-leaf method was used to assess the inhibitory activity of the five alkaloids against TMV. It was found that the alkaloids 1 and 2 exhibited excellent antiviral activity and the both alkaloids were responsible for the antiviral activity of the alkaloid extracts from the alcohol extract of Cynanchum komarovii. The presence of free nitrogen in tertiary amine and phenanthrene ring were essential for high antiviral activity.

关 键 词：生物碱 牛心朴子草 抗病毒活性 烟草花叶病毒 

分 类 号：S482.2[农业科学—农药学]