基质金属蛋白酶抑制剂-1与自发性高血压大鼠心肌纤维化的相关性研究  

The correlation of tissue inhibitor of metalloproteinases - 1 and myocardial fibrosis in Spontaneously Hypertensive Rats

作　　者：闵新文[1] 许明[1] 杨汉东[1] 李东峰[1] 

机构地区：[1]郧阳医学院附属东风医院心内科,湖北十堰442000

出　　处：《中国医师杂志》2007年第6期774-777,共4页Journal of Chinese Physician

摘　　要：目的：探讨基质金属蛋白酶抑制剂-1（TIMP-1）与自发性高血压大鼠（SHR）心肌纤维化的相关性。方法：16只14周龄雄性SHR随机分为卡托普利组和SHR对照组（n=8），8只同龄雄性SD大鼠为正常对照组，卡托普利100mg·kg^-1·d^-1灌胃12周，免疫组化分析TIMP·1、CollagenⅠ和Ⅲ的表达；RT-PCR检测TIMP-1mRNA表达；MASSON染色分析测量胶原容积分数（CVF）；碱水解法测定羟脯氨酸（Hypro）含量。结果：（1）SHR对照组LVI、CVF、Hypro，TIMP-1、CollagenⅠ和Ⅲ表达明显高于正常对照组（P〈0．01）；相对于SHR对照组，卡托普利组则显著降低（P〈0.05）；（2）TIMP-1与上述指标呈正相关（P〈0．01）。结论：TIMP-1与SHR心肌纤维化密切相关，卡托普利能抑制TIMP-1的表达。Objective To evaluate the correlation of TIMP - 1 and myocardial fibrosis in Spontaneously Hypertensive Rats. Methods 16 fourteen week old male SHRs were randomly assigned to captopril and SHR group equivalently （ n =8）. 8 same age male SD rats were selected as control group. A daily dose of 100 mg · kg^-1 · d-l captopril were given for 12 weeks by gavages. TIMP - 1, Collagen Ⅰ and Ⅲ were detected by immunohistochemistry and RT - PCR. CVF was measured by MASSON staining. The concentration of Hypro was detected by alkaline hydrolysis method. Results The LVI, CVF, Hypro, and the expression of TIMP - 1, Collagen Ⅰ and Ⅲ in SHR group were significantly higher than those in SD group （ P 〈0. 01 ）. Compared with the SHR group, these indices in captopril group decreased obviously （ P 〈 0. 05 ）. Conclusions TIMP - 1 closely correlates with myocardial fibrosis in SHR and is suppressed by captopril.

关 键 词：金属蛋白酶1组织抑制剂 高血压 并发症 心内膜心肌纤维化症 病因学 血管肽1 

分 类 号：R544[医药卫生—心血管疾病]