雷公藤多苷对单侧输尿管结扎小鼠肾小管间质纤维化和炎性损伤的影响  

Effects of Tripterygium Wlilfordii on renal interstitial fibrosis and inflamed lesion in UUO mice kidney

作　　者：杨汝春[1] 王永钧[1] 周大为[1] 王军[1] 朱晓玲[1] 

机构地区：[1]杭州市中医院肾病实验室,杭州310007

出　　处：《中国免疫学杂志》2007年第4期322-325,共4页Chinese Journal of Immunology

基　　金：浙江省自然基金资助项目(Y204488);浙江省中医药管理局资助项目(2003C124)

摘　　要：目的通过雷公藤多苷对UUO小鼠肾组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、细胞间黏附分子(Intercellular adhesion molecule1,ICAM-1)、单核细胞趋化因子(Monocyte chem oattractant protein1,MCP-1)表达的影响,探讨其防治肾间质纤维化作用及其机理。方法采用小鼠单侧输尿管结扎(UUO)模型,用雷公藤多苷进行干预,用血管紧张素受体抑制剂蒙诺作为对照。肾脏病理用HE和MASSON染色;肾组织α-SMA表达采用免疫组化;ICAM-1蛋白质表达采用Western blot方法检测;MCP-1基因表达采用逆转录-聚合酶链式反应(RT-PCR)方法。结果UUO模型组小鼠肾间质纤维化程度以及肾组织α-SMA的表达较假手术组显著增高,肾小管间质中炎细胞浸润亦较假手术组明显增加;雷公藤多苷各治疗组肾间质纤维化程度、α-SMA和ICAM-1蛋白、MCP-1基因表达均显著低于模型组;蒙诺治疗亦可显著降低肾间质纤维化程度和肾组织α-SMA的表达,但对于炎细胞浸润和炎症因子的抑制作用不如雷公藤多苷显著,而且未见其抑制ICAM-1表达的作用。结论雷公藤多苷可显著抑制肾间质纤维化和肌成纤维细胞的积聚,其机制可能与其抑制肾组织炎症因子的表达及炎细胞浸润有关。Objective ：To investigate the effects of Tripterygium Wlilfordii on renal interstitial fibrosis and the expression of inflammatory factor in mice kidney with the Unilateral Ureteral Obstruction（UUO）. Methods：Mice were randomly assigned to shame operation group（ shame proup） , UUO group, Tripterygium Wlilfordii and ACEI treatment group after UUO. The protein expression of α- SMA,ICAM-1 were assessed by immunohistochemistry or Western blot; MCP-1 mRNA were detected by reverse transcription-polymerase chain reaction analyses （RT-PCR）. And the change of renal pathology was observed at the same time. Results：In comparison with the shame group, the protein levels of α-SMA, ICAM-1 and the mRNA expression of MCP-1 in UUO mice and treatment groups increased significantly, meanwhile Tripterygium Wlilfordii and ACEI could significantly reduce the expression of α-SMA, ICAM- and MCP-1 on levels of protein and gene. The effects of Tripterygium Wlilfordii on ICAM-1 and MCP-1 were more significant than ACEI. Conclusion：Tripterygium Wlilfordii could reduce the transdifferentiation of renal interstitial ceils and prevent renal interstitial inflammatory injury.

关 键 词：雷公藤多苷 Α-平滑肌肌动蛋白 细胞间黏附分子-1 白细胞趋化因子-1 

分 类 号：R332[医药卫生—人体生理学]