NOD/SCID孕鼠脾脏和胎盘CD45^+细胞内细胞因子的表达  

Intracellular expression of cytokines in CD45^+ cells derived from spleen and placenta of pregnant NOD/SCID mice

作　　者：林羿[1] 陈艺菁[1] 曾山[1] 冯铮[1] 狄静芳[1] 曾耀英[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广州510632

出　　处：《中国免疫学杂志》2007年第4期331-335,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(30672231);中国博士后科学基金(2002032244);广东省自然科学基金(4300213);暨南大学引进优秀人才科研启动基金(51204067)资助

摘　　要：目的研究NOD/SCID孕鼠胚胎吸收率(Resorption rate,RR)与母-胎界面局部免疫状况的关系。方法计算并比较孕13.5天同基因妊娠NOD/SCID×NOD/SCID小鼠和非免疫缺陷的BALB/c×BALB/c小鼠的RR,并采用4色流式细胞术检测NOD/SCID小鼠非孕期和孕13.5天脾脏和胎盘细胞内细胞因子的表达状况,以明确其与妊娠耐受相关的淋巴细胞功能亚群。结果NOD/SCID小鼠的RR与对照组BALB/c小鼠相比无显著差异。与此相应,虽然能够证实NOD/SCID小鼠具有多重免疫缺陷,但是孕期母-胎界面多种功能性细胞亚群的百分率发生自发性改变。结论NOD/SCID小鼠脾脏和胎盘某些细胞百分率的自发性改变可能对妊娠结局有利。Objective：To evaluate the relationship between the embryo resorption rate （RR） and the status of local immunity at the feto-maternal interface in pregnant NOD/SCID mice. Methods： RR was calculated in syngeneically impregnated NOD/SCID × NOD/SCID mice and compared with non-immunodeficiency BALB/c × BALB/c mice at day 13. 5 of gestation. Intracellular expression of cytokines was performed with four-color flow cytometry to identify the functional subsets of splenocytes and placental cells in NOD/ SCID mice before bred or during pregnancy. Results：No statistically supported difference in RRs was observed between NOD/SCID and control BALB/c mice. Accordingly, although multiple immunodeficits were confirmed in NOD/SCID mice, the percentages of several functional cell subsets were spontaneously altered during pregnancy, and this may be correlated with the roughly normal pregnancy outcomes observed in these mice. Conclusion：The spontaneous alteration of cell percentages at the feto-maternal interface in NOD/SCID mice may be beneficial to the pregnancy outcomes.

关 键 词：动物模型 细胞表面标志物 免疫缺陷 妊娠耐受 

分 类 号：R392.32[医药卫生—免疫学]