图像阵列表面等离子谐振生物传感器检测两种磺胺药物  

Imaging Array SPR Biosensor Immunoassays for Sulfamethoxazole and Sulfamethazine

作　　者：李辉[1] 崔大付[1] 梁金庆[1] 刘长春[1] 王于杰[1] 蔡浩原[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室,北京100080

出　　处：《传感技术学报》2007年第7期1479-1482,共4页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助项目(60427001;60302003);国家高技术研究发展计划划(863计划)资助(2006AAO42355)

摘　　要：这项研究利用自制的图像阵列SPR生物传感器对缓冲液中的磺胺甲恶唑(SMOZ)与磺胺二甲嘧啶(SMT)进行了快速灵敏的检测.两种磺胺药物与牛血清白蛋白(BSA)的耦联物被分别固定于阵列芯片上不同的两列,其余一列作为参照.固定过程在仪器中进行,以监控抗原的固定量.抗体与含有磺胺药物的缓冲液混合后流过芯片表面,所得抗体结合曲线的斜率与样品中所含的磺胺浓度成反比例关系.实验中所构建的两种磺胺药物的标准曲线的检测范围在1 ng/mL到15 ng/mL之间.对两种抗体稳定性及特异性进行了研究,单抗对BSA无结合作用.新型高通量、多组份图像SPR生化分析系统为中国科学院电子学研究所自行研制,阵列芯片具有3×5型阵列点.通过使用参考表面,折射率和非特异性吸附的影响可以被去除,从而提高数据的质量.A homemade imaging array surface plasmon resonance （SPR）-based biosensor was used to develop sensitive and fast immolunoassays to determine sulfamethoxazole and sulfamethazine in PBS buffer. Two conjugations of sulfonamide-bovine serum albumin （BSA） were separately immobilized on two different rows of the array chip with the other row as reference. The immobilization was carried out in the instrument to monitor the quantity of the antigen immobilized. The antibody mixed with the sulfonamide in the buffer was injected over the surface of the chip to get a response curve with a slope inversely proportional to the concentration of the sulfonamide in the buffer. Two calibration curves were constructed and the detection scopes were in the range of 1-15 ng/mL. The stability and specificity of the antibody were also studied. The monoclonal antibody didn＇t bind with BSA. The multi-analyte surface plasmon resonance （SPR）-based imaging biosensor was homemade with one channel and the sensor chip had 3 × 5 cells. The change of refractive index and non-specific adsorption could be canceled by using reference surface to improve the quality of the data.

关 键 词：图像 表面等离子谐振 生物传感器 参照表面 磺胺甲恶唑 磺胺二甲嘧啶 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]