RNA Interference-mediated Silencing of Phytochelatin Synthase Gene Reduce Cadmium Accumulation in Rice Seeds  

作　　者：Jiang-Chuan Li Jiang-Bo Guo Wen-Zhong Xu Mi Ma 

机构地区：[1]Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences Beijing 100093, China [2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Journal of Integrative Plant Biology》2007年第7期1032-1037,共6页植物学报（英文版）

基　　金：Supported by the National Program of Research and Development of Transgenic Plants of China （JY03A2001） and the National Natural Science Foundation of China （30570138 and 30370127）.The authors thank Professors Kang Chong and Zheng Meng of the Institute of Botany, the Chinese Academy of Sciences, for kindly providing the vectors of pTCK303 and pKZMM1.

摘　　要：Phytochelatins （PCs） play an important role in heavy metal resistance and accumulation. To reduce the accumulation of cadmium （Cd） in rice seeds, the expression of phytochelatin synthase （PCS） gene OsPCS1 was suppressed by RNA interference （RNAi）. A hairpin construct of a PCS fragment was designed in the pRNAi-OsPCS1 under the control of ZMM1, a seed-specific promoter from maize. The construct was introduced into rice （japonica） through Agrobacterium tumefaciens. The RNAi rice plantlets were selected and cultivated in pots exposured to 10 mg/kg Cd. The transcriptional level of OsPCS1 declined in seeds of some RNAi rice compared to the wild type. As a result Cd accumulation was reduced by about half in the seeds of RNAi rice. As expected, no apparent difference of growth appeared between RNAi and wild-type plants. The results suggest that this new approach can be used to control heavy metal accumulation in crops.Phytochelatins （PCs） play an important role in heavy metal resistance and accumulation. To reduce the accumulation of cadmium （Cd） in rice seeds, the expression of phytochelatin synthase （PCS） gene OsPCS1 was suppressed by RNA interference （RNAi）. A hairpin construct of a PCS fragment was designed in the pRNAi-OsPCS1 under the control of ZMM1, a seed-specific promoter from maize. The construct was introduced into rice （japonica） through Agrobacterium tumefaciens. The RNAi rice plantlets were selected and cultivated in pots exposured to 10 mg/kg Cd. The transcriptional level of OsPCS1 declined in seeds of some RNAi rice compared to the wild type. As a result Cd accumulation was reduced by about half in the seeds of RNAi rice. As expected, no apparent difference of growth appeared between RNAi and wild-type plants. The results suggest that this new approach can be used to control heavy metal accumulation in crops.

关 键 词：ACCUMULATION CADMIUM phytochelatin synthase rice RNA interference. 

分 类 号：S511[农业科学—作物学]