健胃愈疡颗粒对乙酸性胃溃疡复发大鼠作用的蛋白质组学研究  

Proteomic Analysis of the Effect of Jianwei Yuyang Granule on Rats with Recurring Gastric Ulcer Caused by Acetic Acid

作　　者：申定珠[1] 李家邦[1] 蒋荣鑫[1] 凌江红[1] 叶川[1] 马国添[1] 叶仁群[1] 张宗[1] 黄国栋[1] 郭全[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,长沙市湘雅路87号410008

出　　处：《中医杂志》2007年第6期545-548,共4页Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(04JJ3099)

摘　　要：目的分析健胃愈疡颗粒(JWYY)对乙酸性胃溃疡复发大鼠蛋白质表达谱的影响,寻找JWYY抗胃溃疡复发作用的相关蛋白。方法采用二维凝胶电泳(2-DE)分离JWYY处理组和JWYY未处理组大鼠胃组织的总蛋白,图像分析识别差异表达的蛋白质点,基质辅助激光解吸电离飞行时间质谱得到相应的肽质量指纹图,NCBInr及SWISS-PROT数据库搜索鉴定蛋白质。结果所获2-DE图谱分辨率高、重复性好,质谱分析共鉴定了12个差异蛋白质点,其中JWYY处理组有8个蛋白质点表达上调,4个蛋白质点表达下调,大多数蛋白与溃疡复发的形成、生长、凋亡及免疫等环节相关。结论JWYY抗胃溃疡复发作用可能与多种蛋白直接或间接相关,这些蛋白质的鉴定及进一步研究有助于抗溃疡复发药物新型靶标的发现。Objective To study the effect of Jianwei Yuyang Granule （JYG） on protein spectra of rats with recurring gastric ulcer caused by acetic acid and to search the proteins involved. Methods Total proteins in gastric tissue of rats in JYG and nulli-JYG groups were separated by two dimensional gel electrophoresis （2-DE）. Differential protein spots were identified by peptide mass finger- print based on matrix-assisted laser desorption inoization-time of flight mass spectrometry and data searching in National Center for Biotechnology Information and SWISS-PROT databases. Results Well-resolution and reproducible 2-DE maps were obtained and 12 differential protein spots were identified by mass spectrographic analysis. The expression of 8 protein spots increased and 4 decreased in JYG group. Most of the differential protein spots were relevant to formation, proliferation, apoptosis and immunity of the recurrence of gastric ulcer. Conclusion JYG can prevent the recurrence of gastric ulcer, which might be relevant to many proteins. Identification and further study of these proteins conduces to discovering new targets of drugs for ulcer.

关 键 词：健胃愈疡颗粒 胃溃疡复发 蛋白质组 双向凝胶电泳 质谱 

分 类 号：R285.5[医药卫生—中药学]