Ⅱ型胶原诱导的大鼠关节炎模型关节炎症及血清抗体的动态变化  

Changes in serum antibodies and joint inflammation of collagenⅡ-induced arthritis in rats

作　　者：吴荣荣[1] 齐春会[1] 张小锐[1] 程军平[1] 周文霞[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《军事医学科学院院刊》2007年第3期221-224,227,共5页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的：制备Ⅱ型胶原诱导的大鼠关节炎模型（CIA模型）并观察其关节炎症和血清中抗体的变化规律。方法：于第0天和第10天分2次多点皮内注射牛Ⅱ型胶原和完全弗氏佐剂以诱导大鼠CIA模型,应用足爪肿胀容积测定仪动态观察关节肿胀度,采用0~7级关节炎评分法进行关节炎评分,应用组织病理学、X线影像学动态观察踝关节组织的病理变化,应用ELISA方法动态检测血清中多种抗体。结果：CIA模型大鼠免疫后第12天即出现踝关节肿胀,第23天达高峰,持续至第72天（动物处死）;X线检查显示：免疫后第30天,软组织明显肿胀,踝关节骨骼结构排列紊乱,间隙不清,可见囊性骨吸收和骨侵蚀;第60天后踝关节骨骼结构排列更加紊乱,骨质疏松和新骨形成并存,提示侵蚀性慢性关节炎。组织病理学观察显示,第72天踝关节滑膜炎性细胞浸润、滑膜增生,关节软骨及骨破坏,显示较明显的慢性关节炎症。血清中总IgG,IgM型类风湿因子（RF）,IgG型RF和Ⅱ型胶原抗体于免疫后第15天均明显升高,以后维持在较高水平。结论：应用牛Ⅱ型胶原成功制备大鼠CIA模型,免疫后第12天即加强免疫之后即出现关节炎症的病理变化,至少可持续至第72天。Objective： To establish the bovine collagen Ⅱ （C Ⅱ-induced arthritis （CIA）rat model and investigate the pathological characteristics and dynamic changes of serum antibodies. Methods：SD Rats were used to establish CIA model by multi-dot intradermal injection of 150 μg of C Ⅱ emulsified in complete Freund＇s adjuvant（CFA） at the rat tail base on d 0 and d 10, respectively. The degree of swelling and arthritis score were evaluated. The levels of serum antibodies such as IgG, IgG and IgM type rheumatoid factors （ RF）, and anti-C Ⅱntibody were detected by ELISA, Synovial histopathology and imaging changes were observed through roentgenography. Results： The incidence of arthritis in model group was 100% and the peak of joint swelling was achieved on d 23 after immunization. On d 30 soft-tissue swelling and bone destruction were prominent. On d 60 bone destruction was more prominent, periosteal new bone formation appeared, and typical arthritis pathology with chronic proliferative synovitis and secondarily destroyed articular cartilage and bone were found, The serum levels of antibodies such as IgG, IgG RF, IgM RF and anti-C Ⅱantibody were significantly higher than those in the normal control on d 15, and then remained at the higher levels. Conclusion： The CIA rat model was successfully established and the pathological changes of arthritis occurred on d 12 after the first immunization and

关 键 词：类风湿性关节炎 胶原型关节炎模型 组织病理学 抗体 类风湿因子 

分 类 号：R593.22[医药卫生—内科学]