正常犬膀胱组织的显微傅里叶变换红外光谱分析  

Analysis of normal canine bladder tissues by FT-IR microspectroscopy

作　　者：孙东翀[1] 宋占军[2] 陈金龙[1] 梁洁[1] 马萍[1] 杨在富[1] 陈鹏[1] 周游 杨艳峰[1] 高荣莲[1] 钱焕文[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]军事医学科学院国家生物医学分析中心,北京100850 [3]解放军第263医院,北京100101

出　　处：《军事医学科学院院刊》2007年第3期250-253,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的：观察正常犬膀胱组织的显微FT-IR光谱特征,探讨将显微FT-IR技术用于膀胱的组织病理学研究的可行性。方法：制备Beagle犬膀胱标本冰冻切片,在显微FT-IR光谱仪上分别测定黏膜层、黏膜下层及肌肉层的反射吸收光谱,观察不同层次犬膀胱组织的FT-IR光谱特征及其间差异。结果：按光密度值由高到低的顺序,3 302-2 875 cm^-1及1 398-1 086 cm^-1波数区段内依次为黏膜层、黏膜下层及肌肉层,1 669-1 548 cm^-1波数区段内依次为黏膜下层、黏膜层及肌肉层。各层次间差谱的差异主要表现在酰胺Ⅰ、Ⅱ带两处,即黏膜层-黏膜下层的差值为负值,而黏膜层-肌肉层及黏膜下层-肌肉层的差值则均为正值。结论：显微FT-IR技术可以在原位从分子水平上反映正常犬膀胱组织化学组成及其各层次间的差异,有可能为膀胱的组织病理学研究提供一条新的途径。Objective：Fourier transform itffrared （FT-IR） microspectroscopic analyses of different layers of normal canine bladders were carried out in order to investigate the possibility of applying the FT-IR technology to the histological and pathological studies of bladder tissues. Methods： Frozen sections from 18 Beagle canine bladder samples were prepared, and the FT-IR microscopic spectra of mucosa, submucosa and muscle layers were collected in the reflection mode respectively. Characteristics of and differences among the FT-IR spectra from the above three layers were analyzed. Results： In the region of 3 302 - 2 875 cm^-1 and 1 398 - 1 086 cm^-1, absorbance values decreased from [ （ 1. 105〉± 0.099） - （0.583 ±0.026）, （0.611 ±0.019） - （0. 537 ±0.053） ] in the mucosa, to [ （0.948±0.074） - （0. 379 ±0.018）, （0. 597± 0.018） - （0. 222 ±0. 014） ] in the submucosa, and to [ （0. 689 ±0.018 ） - （0. 295 ±0. 014）, （0. 401 ±0. 016 ） - （0. 172 ±0. 017） ] in the muscle layers. In the region of 1 669 - 1 548 cm^ -1 , absorbance values decreased from [ （ 1. 232 ±0.084） - （1.081 ±0. 117 ）] in the submucosa, to [ （ 1.033 ±0.059） - （0.919 ~0.041）] in the mucosa, and to [ （0.951 ±0. 072） - （0. 805 ±0.008） ] in the muscle layers. Significant differences in the subtracted spectra from paired layers （mucosa-submucosa, mucosa-muscle, submucosa-muscle） were the negative peaks of mucosa-submucosa in the re- gions of amide Ⅰ and amide Ⅱ （ 1 669 cm^ -1, 1 548 cm ^-1 ）, in contrast with the positive peaks of the other two pairs in the same regions. Conclusion：Fourier transform-infrared （FT-IR） microspectroscopy could be used for in situ characterization and differentiation of molecular constitutions in the different canine bladder tissue layers, and might be an useful tool for the histological and pathological studies of bladder tissues.

关 键 词：显微傅里叶变换红外光谱 差谱 膀胱 犬 病理学 

分 类 号：R318.51[医药卫生—生物医学工程]