MMP-9、TIMP-1与TGF-β_1在妊娠期高血压疾病患者胎盘组织中的表达及意义  

Research on Expression and Significance of MMP-9,TIMP-1 and TGF-β_1 in Placenta of Patients with Hypertensive Disorder Complicating Pregnancy

作　　者：邱迎春[1] 关咏梅[2] 杨亚滨[1] 孙壮状[2] 

机构地区：[1]哈尔滨医科大学附属第五医院,黑龙江大庆163316 [2]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086

出　　处：《中国初级卫生保健》2007年第7期50-52,共3页Chinese Primary Health Care

摘　　要：目的通过研究基质金属蛋白酶-9(MMP-9)及其抑制物(TIMP-1)与转化生长因子β1(TGF-β1)在妊娠期高血压疾病患者胎盘中的表达,探讨其与本病发病的关系。方法选择2005年11月—2006年11月在我院住院分娩的孕妇63例,分为子痫前期轻度组、子痫前期重度组、妊娠期高血压组、正常妊娠(对照)组,采用免疫组化法检测63例胎盘标本绒毛上皮组织中MMP-9、TIMP-1和TGF-β1的表达。结果①妊娠期高血压患者与正常妊娠者MMP-9的阳性表达,差异无显著性(P>0.05),而子痫前期轻度、重度组MMP-9阳性表达明显弱于正常妊娠组(P=0.012<0.05,P=0.0<0.01);子痫前期TIMP-1的阳性表达略弱于正常妊娠,但无显著性差异(P>0.05);MMP-9/TIMP-1明显下降。②子痫前期重度组胎盘TGF-β1含量明显高于对照组(P=0.019<0.05);子痫前期轻度组、妊娠期高血压组和对照组比较差异无显著性差异(P=0.215>0.05)。③对照组和妊娠期高血压组MMP-9和TGF-β1的表达无相关性(P>0.05);但子痫前期患者,MMP-9和TGF-β1存在明显完全负相关性(r=-1.0,P=0.0<0.01)。结论妊娠期高血压疾病患者MMP-9表达及下降,使得胎盘浅着床,胎盘局部缺血缺氧;TGF-β1表达增多,可能是引发妊娠期高血压疾病的重要原因之一。OBJECTIVE To investigate expression of MMP-9, TIMP-1 and TGF-β1 in human of patients with hypertensive disorder complicating pregnancy for evaluating the role of them in hypertensive disorder complicating pregnancy. METHODS Immunohisochemical staining was used to examine expression of MMP-9, TIMP-1 and TGF-β1 in these samples. RESULTS （1） There is no significant difference on the positive expression of MMP-9 between normal pregnant women and gestational hypertensions （P〉0.05） . The positive expression of MMP-9 in the normal group was slightly higher than that in patients with pre-eclampsia （P=-0.012〈0.05, P=0.0〈0.01）. The positive expression of TIMP-1 in the normal group was slightly higher than that in normal group, but there is no significance （P〉0.05） . And positive expression of MMP-9FFIMP-1 obviously decline. （2）Expression of TGF-β1 in the placenta of patients with severe preeclampsia was significantly higher than that in normal group （P=0.019〈0.05）, but no significance among the patients with mild preeclampsia, gestational hypertensions and normal group （P=0.215〉0.05）. （3）No correlation was found between MMP-9 and TGF-β1 expression in the normal and gestational hypertensions groups （P〉O.05）, but MMP-9 and TGF-β1 expression showed completely negative correlation in the patients with pre-eclampsia （r=-1.0, P=-0.0〈0.01）. CONCLUSIONS Lowed expression of MMP-9 and MMP-9FFIMP- 1 in syncytiotrophoblast might result in defective nidation and ischemia and hypoxia of partial tissue in the placenta. Increased expression of TGF-β1 might be one of the important factors leading to hypertensive disorder complicating pregnancy.

关 键 词：子痫前期 MMP-9 TIMP-1 TGF-β1胎盘 

分 类 号：R714.246[医药卫生—妇产科学]