模拟失重大鼠肺组织损伤发生机制  

THE MECHANISM OF PULMONARY TISSUE DAMAGE IN RATS UNDER SIMULATED WEIGHTLESSNESS CONDITION

作　　者：王俊锋[1] 刘长庭[1] 王德龙[1] 李天智[1] 

机构地区：[1]解放军总医院南楼呼吸科,北京100853

出　　处：《解放军预防医学杂志》2007年第3期164-166,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：总装备部预研基金项目课题(No.51422030101JB1002)

摘　　要：目的探讨模拟失重条件下大鼠肺组织损伤过程中某些细胞因子的变化,以进一步阐述肺组织损伤的内在机制,为临床治疗提供基础。方法采用尾悬吊模拟失重,分为悬吊7 d和21 d及相应对照共4组,每组10只健康雄性SD大鼠。采用原位杂交技术检测肺组织诱导型一氧化氮合酶(iNOS)mRNA、血管内皮生长因子(VEGF)mRNA和诱导型血红素氧合酶(HO-1) mRNA的表达情况,根据染色的程度,由弱至强依次分为0、1、2、3、4级。结果7 d和21 d尾悬吊模拟失重大鼠肺组织iNOS mRNA的表达水平均显著增高,染色为3级的分别是6例和2例,高于相应对照组(P<0.01)。同样,肺组织VEGF mRNA的表达水平显著增高,染色为3级的分别是5例和4例,高于相应对照组(P<0.01,P<0.05)。肺组织HO-1 mRNA表达水平显著增高,染色为3级的分别是5例和6例,高于相应对照组(P<0.01)。结论一氧化氮、VEGF和一氧化碳可能与模拟失重条件下的肺组织损伤有关。Objective To investigate the intrinsic mechanism of pulmonary tissue damage under simulated weightlessness condition. Methods Forty SD male rats were divided into 4 groups： tail-suspension for 7 d, 21 d groups, and corresponding control of 7 d, 21 d groups respectively. Tail-suspension（-30°） was used as weightlessness simulation. The expression of inducible nitric oxide synthase （iNOS） mRNA, vascular endothelial growth factor （VEGF） mRNA and heine oxygenase-1 （HO-1） mRNA in pulmonary tissues was examined by hybridization in situ. According to the degree of staining, it is classified into 5 grades ： 0, 1, 2, 3, 4. Results The expression level of iNOS mRNA in pulmonary tissue of 7 d, and 21 d tail-suspended rats was significantly increased, for example, the positive staining number of rats in the 3 rd grade was 6, and 2 respectively, whereas that in corresponding control rats was 0（ P 〈 0.01）. Similarly, the expression level of VEGF mRNA, and HO-1 mRNA was significantly increased as well. For VEGF mRNA, the positive staining number of rats in the 3 rd grade was 5,4 vs 0（ P 〈 0.01, P 〈 0.05） ; for HO- 1 mRNA, 5,6 vs 0 （ P 〈 0.01 ）. Conclusion Nitric oxide, vascular endothelial growth factor and carbon monoxide may be related to pulmonary tissue damage under simulated weightlessness condition.

关 键 词：模拟失重 一氧化氮 血管内皮生长因子 一氧化碳 肺组织 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]