一种能原位检测类雌激素暴露的胶体金免疫检测试片  

An Immunochromatographic Test Strip for in situ Identification of Vitellogenin Induction in Fish Exposed to Xenoestrogens

作　　者：杨方星[1] 徐盈[1] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072

出　　处：《生态毒理学报》2007年第2期220-224,共5页Asian Journal of Ecotoxicology

基　　金：科技部863项目(No.2003AA646010;No.2006AA06Z424)

摘　　要：利用免疫层析和胶体金标记技术试制出一种免疫检测试片,该试片可快速定性检测鱼体内诱导的卵黄蛋白原.试验结果表明,该检测试片仅需采鱼血浆100μL,15min内便可获得检测结果.试片的检测限(LODVTG)为10μg·mL-1,野外现场应用表明测量准确度达92%.因此,该试片可用于野外水体类雌激素暴露与影响的原位评价.An immunochromatographic test strip based on gold colloid was developed to quickly identify vitellogenin （VTG） induction in fish in field waters. The results indicated that the immunochromatographic test strip could perform the analysis with 100μL plasma in 15min. The limit of detection （LODVTG）of the immunochromatographic test strip was 10μg· mL^-1 and the accuracy reached 92%. Therefore, it is proposed that the test strip can be a primary means for in situ assessing the effect of xenoestrogen exposure.

关 键 词：胶体金 卵黄蛋白原 类雌激素 检测试片 

分 类 号：Q57[生物学—生物化学]