mRNA差别显示技术及其在生命科学中的应用  

Method of mRNA Differential Display and Its Application in Life Science

作　　者：徐国恒[1,2] 凌雁[1,2] 万有[1,2] 王晓民[1,2] 

机构地区：[1]北京医科大学神经科学研究中心 [2]河南医科大学病理学教研室

出　　处：《生理科学进展》1997年第1期19-23,共5页Progress in Physiological Sciences

摘　　要：ｍＲＮＡ表达水平的变化决定细胞的功能状态。个体发育、细胞增殖分化与凋谢、生理刺激和药物治疗等过程，都会出现ｍＲＮＡ表达水平的变化。阐明这种变化有助于揭示细胞生理过程的分子机制。新近发展起来的ｍＲＮＡ差别显示技术为此提供了强有力的工具。该技术无需更多的背景资料，可快速有效地分离表达水平出现差别的基因。The changes of mRNA expression level control cell function. Many factors such as individual development, cell proliferation and differentiation, and even apoptosis, physiological stimuli, and drug treatment will change the level of gene expression. Comparisons of gene expression in different cell types provide the underlying information for analyzing the molecular and cellular mechanisms of the physiological processes. A method of mRNA differential display is developed recently, providing a powerful and useful tool, which can efficiently and rapidly isolate the genes that display a difference in transcription. These genes may play important role in cell function.

关 键 词：MRNA差别显示 生命科学 方法技术 

分 类 号：Q522.03[生物学—生物化学]