LHRH刺激雄性大鼠垂体LH分泌高峰时细胞形态变化的图象分析  

AN IMAGE ANALYSIS OF THE MORPHOLOGICAL CHANGES OF LH CELLS DURING LH RELEASE PEAK INDUCED BY LHRH IN MALE RATS

作　　者：周寿康[1] 赵伟[1] 毛全福[1] 唐威[1] 何志英[1] 顾敦瑜[1] 蒋文珏[1] 

机构地区：[1]上海市计划生育科学研究所,上海200032

出　　处：《生理学报》1997年第1期88-94,共7页Acta Physiologica Sinica

基　　金：国家自然科学基金!39370279

摘　　要：应用ABC亲和组织化学顺序、MIS－I计算机图象处理系统测定外源性LHRH刺激雄性大鼠LH分泌高峰前后LH细胞面积、细胞内液泡面积和细胞形状等形态参数的变化。结果表明：雄性大鼠LH基础分泌时，LH细胞处于贮存状态：大细胞（＞340μm2）占LH阳性细胞56．7％，小细胞（＜190μm2）占2．2％，40％细胞内含有大量液泡，细胞形状大多偏向圆形。注射LHRH30min后，血中LH浓度显著升高，LH细胞平均面积明显减小；60min时，血中LH释放达到高峰，相应的形态学特征是大细胞降低至4％，小细胞增加到66％。随着细胞内液泡的大量消失，52．6％的细胞偏向不规则形状。LH分泌高峰后，血液LH浓度逐渐恢复到基础分泌水平，但随着细胞内LH贮存的增加，细胞和细胞内液泡平均面积逐渐增加，大多数细胞又偏向圆形。结果提示，合大量液泡的偏圆形大细胞同LH大量贮存有关。而液泡的消失以及大量小细胞和不规则形状细胞的出现同LH大量释放有密切联系。由此作者认为：LH细胞内液泡的动态变化以及由此产生的细胞大小、细胞形状和细胞结构的变化可以作为促性腺激素分泌的重要形态学观察指标。The relationship between the pek of LH release induced by exogenous LHRH and morphological change of LH cells in male rats was invedigated by ABC Affinity histochemistry and qualitutively characterized by Medical Image Processing System-I. The serum LH concentrati0n was determined by RIA. During the Period of basal secretion of LH (3. 76± 0. 39 ng/ml), the LH cellsmight be talgned at a storage state. Most of such cells (56. 7% ) are round and large, and theircross sectional areas are larger then 340μm2, usually contaling large vacuoles, while the smallercells of X-sectional area less then 190 μm2 counted only 2% of the totul population. Thirty minutdsafter injection of LHRH the serum th level increased significantly (6. 46±1. 6 ng/ml, P＜0. 01 ), there appeared a lot of small angular cells, the average cross sectional areas of LH cells decreased significantly (P＜ 0. 0001 ). When LHRH was injected at sixty minutes, the LH levelreached a peak (18. 78±0. 79 ng/ml), the number of the large cells decreased to only 4 %, whilethe srnall cells incred to 66%, vacuoles were almost disapperanced and a large pert of cells(52. 6 % ) became irregular. Thereafter, the LH serum gradually subsided to itS original basal levelwith attendant resumption of the morphology of the LH cells at storage stute. It appers that the morphological changds, such as size, shape, vacuoles and so on, of the LH cells may serve as an important index for showing the effect of LHRH on LH secretion.

关 键 词：内分泌生理 垂体 LH LHRH 细胞 形态 

分 类 号：R335.1[医药卫生—人体生理学]