生脉散对自由基致大鼠离体心脏损伤保护作用  

Protective effects of Shengmaisan on isolated heart injury induced by free radical in rats

作　　者：刘红[1] 艾明仙[1] 阳辉[1] 

机构地区：[1]湖北民族学院医学院药理教研室,恩施445000

出　　处：《中国公共卫生》2007年第7期847-849,共3页Chinese Journal of Public Health

基　　金：湖北省卫生厅科研基金(JXIB120)

摘　　要：目的观察对家兔灌胃给予生脉散后,其含药血清对大鼠离体灌流心脏内外源性自由基所致心肌损伤的保护作用。方法家兔每日2次灌胃给予生脉散5 ml/kg,连续3 d,末次给药后60 min取血,分离血清。将含药血清加到Langendorff法灌流的大鼠离体心脏,记录左室内压(LVP)、左室内压最大上升速率(+dp/dtmax)、左室舒张末压(LVEDP)、心率(HR),定时收集冠脉流出液测定冠脉流量(CF)和肌酸激酶(CK)活性,再灌结束时测定心肌组织中丙二醛(MDA)含量。结果以含0.25μmol/L 1.1-二苯基-2-三硝基苯肼(DPPH)或0.06μmol/L次黄嘌呤(HX)和2.0μ/L黄嘌呤氧化酶(X0)的K-H液灌流心脏10 min,可显著降低LVP、+dp/dtmax升高LVEDP,增加CK和MDA释放;预先用含药血清灌流心脏10 min,可显著改善DPPH或HX+XO所致心功能损伤,减少CK和MDA释放。结论含生脉散血清对DPPH或HX+XO诱导内外源性自由基所致心肌损伤具有明显保护作用。Objective To investigate the protective effects of Shengmaisan on myocardial injury induced by endogenous and exogenous free radical in isolated rat hearts. Methods The experiment was accomplished by serum pharmacology. All rabbits were treated with Shengmaisan via gastric gavage twice daily for three days consecutively. After this treatment, 60 rain after the last dosing the blood was taken and the serum was separated. The herbal medicines serum was added to the isolated rat hearts perfused in a Langendorff model. Left ventricular pressure（LVP）, the frist derivative of LVP（ ＋ dp/dtmax）, left ventricular end-diastolic pressure（LVEDP）and heart rate（ HR） were recorded. Coronary flow（CF）in coronary effluent were measured by timed collection of coronary effluent, at the end of the reperfusion, the content of malondialdahycle（MDA） in cardiac tissue was measured. Results Perfusion withl, 1-diphenyl-2-picryl-hydrazyl （DPPH 0.25 μmol/L） or hypoxanthine （HX0.06 μmol/L） and xanthine oxidase （XO 2.0 u/L）for 10 min caused a marked decrease in LVP and ＋ dp/dtmax, and an increase in LVEDP, the release of CK and the content of MDA. pretreatment with herbal medicines serum for 10 rain produced a significant improvement of cardiac function injury induced by DPPH or HX ＋ XO and a decrease in the release of CK and the content of MDA. Conclusion Shengmaisan marked protectively effects on myocardiac injury induced by endogenous and exogenous free radical which were produced by DPPH or HX ＋ XO.

关 键 词：生脉散 自由基 心脏 

分 类 号：R285.5[医药卫生—中药学]