镉致大鼠肾脏毒性机制研究  

Study on mechanism of toxicity induced by cadmium in kidney

作　　者：杨敬华[1] 徐兆发[1] 徐斌[1] 贺安宁[1] 

机构地区：[1]中国医科大学公共卫生学院环境卫生学教研室,沈阳110001

出　　处：《中国公共卫生》2007年第7期887-888,共2页Chinese Journal of Public Health

基　　金：国家自然基金项目(30371200)

摘　　要：目的研究镉对肾脏产生的毒性作用,并初步探讨镉的肾脏毒性机制。方法低、中、高剂量染镉组大鼠分别皮下注射3,5,7μmol CdCl2/(kg.bw)6周。测定尿镉、蛋白含量和尿N-乙酰-β-D氨基葡萄糖苷酶(NAG)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)活性,测定肝脏和肾皮质丙二醛(MDA)和谷胱甘肽(GSH)含量。结果尿蛋白含量和尿NAG、ALP、LDH活性以及肝、肾皮质MDA和GSH含量随染镉剂量增加而升高。中剂量染镉组尿蛋白含量和尿NAG、LDH活性以及肾皮质MDA、GSH含量显著高于对照组和低剂量染镉组。高剂量染镉组尿蛋白含量、ALP活性、肝和肾皮质MDA显著高于对照组和低中剂量染镉组。且尿镉、蛋白含量、尿NAG、ALP、LDH活性与肾皮质MDA含量呈显著正相关。结论镉生物学效应随染毒剂量增加而增强,CdCl25μmol/(kg.bw)对肾脏产生一定损伤作用,CdCl27μmol/(kg.bw)时肾脏损伤加重。镉的肾毒性机制与其氧化损伤作用有关。Objective To study toxic effects of cadmium （Cd） on the kidney, and discuss the mechanism of toxicity induced by Cd in kidney. Methods Three Cd groups rats were injected with CdCl2 3, 5 and 7 μmol/kg per clay for 6 weeks, respectively. Urinary Cd, protein contents and N - acetyl - β - D - glucosaminidase （NAG）, alkaline phosphalase （ALP）, lactic dehydrogenase （LDH） were measured. Malondialdehyde （MDA）, glutathione （GSH） contents in the liver and renal cortex were determined. Results Urinary Cd, protein contents, NAG, ALP, LDH activities and MDA, GSH contents in the liver and renal cortex increased with Cd dose increasing. Urinary protein contents, NAG, LDH activities and MDA, GSH contents in the renal cortex of middle - dose Cd group were higher significantly than those of control and low - dose Cd group. To high - dose Cd group, urinary protein contents, ALP activities and MDA contents in the liver and renal cortex were higher significantly than that of control, low - and middle - dose Cd group. Urinary Cd, protein contents and NAG, ALP, LDH activities were correlated positively with MDA contents in the renal cortex. Conclusion The biological effect of Cd enhanced with Cd dose increasing. CdCl2 5μmol /（kg·bw）could induce renal injuries, and CdCl2 7μmol /（ kg·bw）could induce more evident injuries. Furthermore, the mechanism was correlated positively with oxidative injuries caused by Cd.

关 键 词：镉 肾脏 

分 类 号：R994.6[医药卫生—毒理学]