日本血吸虫28kD GST基因在家蚕细胞和幼虫中的表达  

Studies on the Expression of the 28kD Giutathione S-transferase Gene from Schistosoma japonicum in the Silkworm(Bombyx mori) Larvae and Insect Cells

作　　者：田锷[1] 杨冠珍[2] 蔡幼民 施福恢[1] 吴祥甫[2] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,200232 [2]中国科学院上海生物化学研究所,200031

出　　处：《生物化学与生物物理学报》1997年第1期33-39,共7页

基　　金：总理专项基金(94-y-40)资助项目

摘　　要：应用修饰的家蚕核型多角体病毒为载体在家蚕细胞和幼虫中表达日本血吸虫中国大陆株２８ＫＤ胱甘肽Ｓ－转移酶基因。Ｓｏｕｔｈｅｒｎ杂交证实该基因被插入到病毒基因组中的正确位置。在家蚕培养细胞中的产量为０．７７ｍｇ，在家蚕幼虫中则超过５ｍｇ／条蚕。The gene of the 28kD glutathione S-transferase(GST) from the Chinese strain of Schistosoma ja ponicum had been expressed in the silkworm (Bombyx mori ) cells and larvae by Bombyx mori nuclear polyhedrosis virus (BmNPV) vector which had been modified. The GST gene was inserted into the right position of the BmNPV genome as identified by Southern hybridization. The product was a 28kD protein, had GST activity and antigenicity. The yield in BmN cells(1×10~6 cells/ml) was 0.77mg and more than 5mg in a silkworm larvae. All the results showed that it is possible to develop the GST expressed in the silkworm larvae to a new kind of genetic engineered schistosomiasis vaccine.

关 键 词：家蚕 谷胱甘肽S 转移酶 日本血吸虫 基因表达 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q753[农业科学—畜牧兽医]