谷胱甘肽产生菌的菌种选育  

Breeding of Glutathione-Overproducing Strain

作　　者：陈雪[1] 赵文杰[1] 冯军[1] 程晴华[1] 薛春佳[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》2007年第7期481-483,共3页Chinese Journal of Pharmaceuticals

摘　　要：采用紫外线和亚硝基胍对出发菌株SIPI-G0619进行诱变,同时使用乙硫氨酸、氯化锌和三氮唑三种抗性筛选物为筛选模型,最终筛选得到稳定的谷胱甘肽高产菌株SIPI-Gh435;谷胱甘肽产量为864mg/L,是出发菌株的4.9倍。Candida utilis SIPI-G0619 was mutagenized by ultraviolet radiation and NTG treatment. A new mutant strain SIPI-Gh435 was finally isolated under the coaction of ethionine, zinc chloride and 1,2,4-triazole. The yield of intracellular glutathione in SIPI-Gh435 was 864mg/L, which was 4.9 times higher than the original strain.

关 键 词：假丝酵母 谷胱甘肽 菌种选育 

分 类 号：Q933[生物学—微生物学]