睾酮转化菌的诱变育种  

Mutation and Screening of Testosterone Producing Strain

作　　者：戈梅[1] 夏兴[1] 凌良飞[2] 陈代杰[1] 

机构地区：[1]上海医药工业研究院 [2]南京农业大学微生物系,江苏南京210095

出　　处：《中国医药工业杂志》2007年第7期488-490,共3页Chinese Journal of Pharmaceuticals

摘　　要：从雄甾二酮的转化产生菌偶发分枝杆菌MF2出发,经紫外诱变结合平板筛选,获得一株主要转化生产睾酮的突变株TS-N-121。该菌株可以胆固醇等单一或混合甾醇作为原料,经一步培养转化生产睾酮,其含量占总转化产物的90%以上,转化率大于80%。A mutant, TS-N-121 was obtained by mutation and plate screening from a parent strain Mycobacterium fortuitum MF2, which could transform sterol into androstanedione as the main product. This mutant strain could also be used to transform single or mixed sterols into testosterone by a one-step microbial cultivation. The bioconversion efficiency was over 80 %, while the content of testosterone in the total transformation products exceeded 90 %.

关 键 词：睾酮 生物转化 甾醇 偶发分枝杆菌 

分 类 号：Q933[生物学—微生物学]