甜瓜RAPD反应体系的优化  

Optimization of RAPD Reaction System in Cucumis melo

作　　者：柯妍柠[1] 刘莉[1] 李秀秀[2] 郭富常[1] 张秦英[1] 

机构地区：[1]天津大学农业与生物工程学院,天津300072 [2]天津科润蔬菜研究所,天津300384

出　　处：《天津农业科学》2007年第2期7-10,共4页Tianjin Agricultural Sciences

基　　金：天津市重点科技攻关专项资助项目(05YFGZNC01304)

摘　　要：以甜瓜单性花类型植株为材料,采用改良的CTAB法提取基因组,对影响RAPD反应的各种因子进行筛选。结果表明:在20μL反应体积中,RAPD优化反应体系为:模板DNA浓度2.5ng/μL,dNTP 0.25 mmol/L,随机引物0.8μmol/L,TaqDNA聚合酶1.0U。利用此优化的反应体系,采用BSA法筛选出一条长约550bp与甜瓜单性花相关基因连锁的分子标记。Modified CTAB was used to extract genomie DNA from Cucumis melo L. Each factor in RAPD reaction system was studied. The optimal PCR system for RAPD analysis was as follows： the genomie DNA concentration was 2.5ng/ul, dNTP concentration was 0.25mmol/L, primer concentration was 0.80umol/L as well as Taq DNA polymerase concentration was 1U in 20ul reaction system. Based on this optimal PCR system, a dominant marker, 550bp, was found using bulked segregation analysis strategy （BSA）.

关 键 词：甜瓜 RAPD 优化 

分 类 号：S652[农业科学—果树学]