利用电化学及光谱方法研究环丙沙星与DNA的相互作用  

The Electrochemical behavior of Ciprofloxacin and Its Interaction of Ciprofloxacin and ctDNA by Electrochemical and Spectroscopy Techniques

作　　者：赵长庆[1] 钱彬彬[1] 蔡跃娟[1] 张玉忠[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2007年第4期477-480,共4页Journal of Anhui Normal University(Natural Science)

基　　金：国家自然科学基金(20675002)

摘　　要：用循环伏安法研究了环丙沙星在单壁碳纳米管(SWNT)修饰电极上的电化学行为,在pH5.0的磷酸盐缓冲溶液(PBS)中,环丙沙星只出现一个氧化峰,峰电位为1.439 V.与其在裸玻碳电极相比,环丙沙星在单壁碳纳米管修饰电极上峰电流有明显增强;若底液中存在DNA时,环丙沙星峰电流减小,峰电位正移.另外用紫外和荧光光谱研究了DNA与环丙沙星的相互作用.结果表明其紫外吸收强度减弱,吸收峰红移,相对荧光强度减弱.实验结果表明环丙沙星与DNA之间形成新的加合物.Cyclic Voltammetry coupled with fluorescence and UV/Vis absorbance spectra techniques were used to study the interaction of ciprofloxacin and calf thymus DNA in phosphate buffer solutions. The interaction of ciprofloxacin and ctDNA could result a considerable decrease in the peak currents and positive shift in the peak potential, decrease in the adsorption intensity and red shift in the adsorption peak, and decrease in the fluorescence emission. The experiment results showed that the new adduct between ciprofloxacin and ctDNA was formed.

关 键 词：环丙沙星 CTDNA 单壁碳纳米管 修饰电极 荧光光谱 紫外光谱 

分 类 号：O657.1[理学—分析化学]