软肝冲剂抑制血管紧张素Ⅱ诱导的肝星状细胞上调基因的表达  

Ruangan granule prevents up-regulation genes angiotensin Ⅱ-induced in hepatic stellate cells (HSCs)

作　　者：莫其农[1] 黄涛[1] 方晓艺[2] 周小梅[1] 何进才[1] 贺劲松[3] 周小舟[3] 童光东[3] 熊益群[3] 周大桥[3] 

机构地区：[1]广州中医药大学附属深圳市中医院检验科,广东深圳518033 [2]广州中医药大学附属深圳市中医院体检中心 [3]广州中医药大学附属深圳市中医院肝病研究所

出　　处：《中西医结合肝病杂志》2007年第3期147-150,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：深圳市科技局立项项目(NoJH200507130998A)

摘　　要：目的:旨在研究软肝冲剂是否通过抑制血管紧张素Ⅱ(AngiotensinⅡ,AngⅡ)诱导的肝星状细胞(HSCs)上调基因起作用。方法:用DNA微点阵分析用AngⅡ(10-8M)以及AngⅡ和软肝冲剂(2.5mg/L)处理的HSCs与对照组之间基因的差异性表达,其结果用实时定量RT-PCR证实。用Westernblot分析软肝冲剂对HSCs在AngⅡ诱导下的胶原蛋白的表达,ELISA分析有生物活性的TGF-β1的含量。结果:软肝冲剂能抑制AngⅡ诱导HSCs上调基因的表达,特别是一些细胞外基质和细胞因子如IL-13,IL-15,MCP-2,TGF-1,TGF-2,细胞因子受体基因,如TGF-1受体。分泌到细胞培养基中的胶原蛋白和有生物活性的TGF-β1也被抑制。结论:软肝冲剂抑制AngⅡ诱导HSCs上调的基因的表达,这可能是软肝冲剂治疗肝纤维化和肝硬化的机制之一。Objective： To study whether Ruangan granule can inhibit up-regulated genes in HSCs AngⅡ induces. Methods： Differently expressing genes were compared between AngⅡ （10^-8M）, AngⅡ （10^-8M） plus Ruangan granule （2. 5mg/ L） group and untreated one via DNA microarray analysis, and the results of DNA microarray analysis were demonstrated by Real-time RT-PCR. Collagen expression and bioactive TGF-β1 were measured by Western blot and ELISA respectively in HSCs Ruangan granule in combination with AngⅡ induced. Results： Ruangan granule could inhibit the expression of up-regulated genes in HSCs AngⅡ induces, in particular, some extraeellular matrix, eytokines, e. g. IL-β, IL-15, MCP-2, TGF-1, 2 and eytokine receptors, e. g. TGF-1 receptor. Collagen and bioactive TGF-β1 HSCs secreted in medium were also inhibited by Ruangan granule. Conclusion： Ruangan granule prevents up-regulation genes Angiotensin Ⅱ-induced in HSCs, which might be one of mechanisms it can treat liver fibrosis and liver cirrhosis.

关 键 词：DNA微点阵 软肝冲剂/药效学 血管紧张素Ⅱ 肝星状细胞 肝硬化 

分 类 号：R285[医药卫生—中药学]