灰飞虱携带水稻条纹叶枯病毒检测研究  

Research on detecting rice stripe virus in Laodelphax striatellus

作　　者：孙祥良[1] 王华弟 朱金良[3] 曹奎荣[1] 赵建平[1] 戴德江 

机构地区：[1]浙江省嘉兴市农业科学研究院,浙江嘉兴314016 [2]浙江省植物保护检疫局,杭州310020 [3]嘉兴市农业经济局,314050

出　　处：《中国植保导刊》2007年第7期7-9,共3页China Plant Protection

基　　金：浙江省重大科技攻关项目(2004C12023);嘉兴市科技发展项目(2005AZ3002-2)

摘　　要：采用斑点免疫结合(DIBA)与生物测定方法对采自浙江长兴和嘉兴两地的灰飞虱带(传)毒率进行了检测。结果发现,两地灰飞虱均有部分虫口携带水稻条纹叶枯病毒(RSV),平均带毒率为6.15%,传毒率为3.96%;斑点免疫结合(DIBA)测定的灰飞虱带毒率,均高于生物法测定的灰飞虱传毒率,两者之比为1:0.65。该检测结果为病害预测和防控提供了重要科学依据。Rice stripe virus （RSV） in Laodelphax striateUus was detected through the dot-immunobinding assay （DIBA） and bioassay. Results revealed that 6.15% Laodelphax striateUus infected with virus, mean transmission rate was 3.96%. The rate of Laodelphax striateUus which infected by virus detected by DIBA was higher that that by the bioassay, the ratio of Laodelphax striateUus infecting virus detected by DIBA to that by bioassay was 1：0.65. These results provided important scientific evidences for forecasting and controlling Laodelphax striatellus.

关 键 词：条纹叶枯病 灰飞虱 带毒率 检测 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]