反义4CL与UGPase双价基因在烟草中的转化及表达分析  

Vector Construction with UGPase and anti4CL, and Their Expression in Transgenic Tobacco

作　　者：梁海泳[1] 夏秀英[1] 高晓蓉[1] 苏乔[1] 

机构地区：[1]大连理工大学环境与生命学院,大连116026

出　　处：《植物学通报》2007年第4期459-464,共6页Chinese Bulletin of Botany

基　　金：辽宁省博士启动金(No.20031080)

摘　　要：构建了携带紫穗槐尿苷二磷酸葡萄糖焦磷酸化酶(UDP-glucose pyrophosphorylase,UGPase)基因和4-香豆酸:辅酶A连接酶(4-coumarate:coenzyme A ligase,4CL)反义基因的双价基因植物表达载体,用热激法转化至根癌农杆菌LBA4404中,并用制备的农杆菌工程菌进行了烟草转化。PCR及Southern杂交结果证实,双价基因已成功整合到烟草基因组中。综纤维素和硫酸木质素含量测定结果显示,转基因烟草纤维素含量增加,木质素含量减少,表明双价基因能够有效表达。We describe our construction of a plant expression vector, which contained an UDP-glucose pyrophosphorylase gene and an anti-sense 4-coumarate： CoA ligase gene. The vector was transferred by use of Agrobacterium tumefaciens into tobacco. Results of PCR and Southern blot analysis indicated that both genes were integrated into the tobacco genome successfully. Holocellulose content was increased and Klason lignin content significantly reduced, which illustrated that the two genes were expressed effectively in the tobacco genome.

关 键 词：纤维素 辅酶A连接酶 4-香豆酸 木质素 尿苷二磷酸葡萄糖焦磷酸化酶 双价基因 

分 类 号：Q943.2[生物学—植物学]