生物反应器在满天星快繁中的应用  

Application of Bioreactors in Rapid Propagation of Gypsophila paniculata

作　　者：张春华[1] 朴炫春[1] 廉美兰[1] 王守明[1] 

机构地区：[1]延边大学农学院园艺系,龙井133400

出　　处：《植物学通报》2007年第4期526-531,共6页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30560094);国家教育部科学技术重点项目(No.205035)

摘　　要：利用2.5L柱状气升式生物反应器采用接触法进行了满天星(Gypsophila paniculata)增殖培养。结果表明:外植体接种密度(每个反应器接种外植体个数)为35比密度为25和45更有利于满天星的增殖生长,平均每个生物反应器内可获得252株生长健壮的不定苗;光强为90μmol.m-2.s-1对满天星增殖培养最有利,过高的光强对不定芽和株高有抑制作用;通过小孔隙(15μm)的多孔喷头注入0.1vvm空气有利于满天星增殖培养。We investigated proliferation culture of Gypsophila paniculata by using a raft method in a 2.5 L air-lift bioreactor of the column type. Inoculation density of 35 explants in the bioreactor was more favorable than treatments of 25 and 45 explants for proliferation; 252 vigorous adventitious shoots were obtained for the 35 explants with the bioreactor. A light intensity of 90 μmol·m^-2·S^-1 was considered the most effective, but excessive light inhibited shoot formation and length. Furthermore, proliferation of G. paniculata in the bioreactor was more efficient with lacunaris sparger of small pore size （15 μm） and 0.1 vvm of air.

关 键 词：生物反应器 满天星 增殖培养 

分 类 号：S681.9[农业科学—观赏园艺]