Phylogenetic relationships of host insects of Cordyceps sinensis inferred from mitochondrial Cytochrome b sequences  

作　　者：Cheng Zhou Geng Yang Liang Honghui Yang Xiaoling Li Shan 

机构地区：[1]School of Life Science and Technology, Tongji University, Shanghai 200092, China [2]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, China

出　　处：《Progress in Natural Science:Materials International》2007年第7期789-797,共9页自然科学进展·国际材料（英文版）

基　　金：Supported by the Special Plan of Modernization of Traditional Chinese Medicine from Shanghai Municipal Science and Technology Commission(03DZ19547)

摘　　要：This study used the sequence of the mitochondrial Cytochrome b （Cytb） to estimate phylogenetic relationships among host Hepialidae insects of Cordyceps sinensis. Genome DNA of host insect was extracted from the dead larva head part of 18 cordyceps populations and 2 species of Hepialus, and the Cytb fragment of host insect was amplified with PCR technique. The nucleotide sequence alignments and their homologous sequences of 24 species host Hepialidae insects of Cordyceps sinensis were obtained from GenBank and were used to construct phylogenetic trees based on neighbor-joining method. The results showed that genus Bipectilus diverged earlier than genus Hepialus and Hepialiscus. Hepialus host insects of Cordyceps sinensis have multitudinous species with different morphological characteristics and geographical distributions. The interspecific genetic differentiations are obvious in Hepialus. Thus, the genus Hepialus might he considered as polyphyletic origin. Cytb sequences have abundant variations among the host insects of Cordyceps sinensis on specific and generic level. The divergence rate of Cytb sequences among the species in Hepialus ranged from 0.23% to 9.24%, except that Hepialus pratensis and Hepialus jlnshaensis have the same sequence. Cytb sequence can be used for species identification of host insects of Cordyceps sinensis, hut further confirmation in more host insect species is needed. To obtain the Cytb sequence of host insect by amplifying DNA extracted from the head part of dead larva in cordyceps turns out to he an effective and accurate approach, which will he useful for studies on phylogeny and genetic structure of host insects of cordyceps populations, especially for analyzing relationships between C. sinensis and its host insects.这研究使用了 mitochondrial 细胞色素 b (Cytb ) 的顺序在 Cordyceps sinensis 的主人 Hepialidae 昆虫之中估计种系发生的关系。宿主昆虫的染色体 DNA 从 18 张 cordyceps 人口的死了的幼虫的头部分被提取， Hepialus 的 2 种类，和 Cytb 宿主昆虫碎裂与 PCR 被放大技术。核苷酸顺序排列和他们 Cordyceps sinensis 的 24 只种类主人 Hepialidae 昆虫的相应序列从 GenBank 被获得并且被用来基于加入邻居的方法构造种系发生的树。结果证明类 Bipectilus 比类 Hepialus Hepialiscus 早分叉。Cordyceps sinensis 的 Hepialus 宿主昆虫与不同词法特征和地理分布有大群的种类。种间的基因区别在 Hepialus 是明显的。因此，类 Hepialus 可能被看作 polyphyletic 起源。Cytb 序列在特定、通用的水平上在 Cordyceps ninensis 的主人昆虫之中有丰富的变化。在在 Hepialus 的种类之中的 Cytb 序列的分叉率从 0.23% ～ 9.24%，除了 Hepialus pratensis 和 Hepialus jinshaensis 有一样的顺序之外。Cytb 顺序能被用于 Cordyceps sinensis 的主人昆虫的种类鉴定，但是推进证实在在先，主人昆虫种类被需要。为了由放大 DNA 获得主人昆虫的 Cytb 顺序，外面从在 cordyceps 拐弯幼虫的死者的头部分提取了是有效、精确的 apprach ，它将为发展史和 cordyceps 人口的主人昆虫的基因结构上的研究是有用的，特别为分析在 C.sinensisand 之间的关系它的主人昆虫。

关 键 词：mitochondrial Cytochrome b Cordyceps sinensis HEPIALUS phylogeny. 

分 类 号：Q969.42[生物学—昆虫学]