用RAPD技术测定黑荆树种源遗传关系初报  

The Variability among Provenances of Acacia mearnsii Detected by RAPD

作　　者：黄少甫[1] 李桂英[1] 赵治芬[1] 陆军[2] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,副研究员浙江富阳311400 [2]中国水稻研究所

出　　处：《林业科学研究》1997年第1期86-89,共4页Forest Research

基　　金：中国林业科学研究院1995基金项目"林木抗性遗传机理研究"的内容

摘　　要：黑荆树(Acacia mearnsii Willd)系含羞草科(Mimosaceae)金合欢属(Acacia)植物,是一多用途、速生树种。我国引种时间较短,种源混杂,但黑荆树象其它树种一样,表型的变化受环境影响极大,种内各品种之间的差异很小,很难用常规的方法加以识别和划分。而应用RAPD技术可以快速、简便、灵敏地检测DNA的多态性,将品种间的差异显著地反映出来。这已在医学上、农业上得到广泛的应用。RAPD在林业上也已有应用,例如,苏晓华用RAPD技术估测了柳树种及无性系的变异;李宽鈺利用RAPD分子标记对白杨派。The genomes of 11 provenances of Acacia mearnsii Willd were tested by RAPD with 22 random primers. It could be seen from the electrophoretogram of agarose gel that different primers amplified DNA bands ranging from 1 to 7, and that primer 428 ampli- fied only 1 band and primer 17 amplified 7 bands. The dendrogram was established based on the DNA bands of the amplified products which showed diversity among different prove- nances. The results showed no significant correlations between provenances' hardiness with their geographical longitude, latitude or elevation.

关 键 词：黑荆树 RAPD技术 种源 遗传 

分 类 号：S792.990.4[农业科学—林木遗传育种]