胡颓子多糖对小肠辐射损伤的保护作用  

Effects of Elaeagnus bockii Diels polysaccharide on the small intestine of mice after whole body irradiation with ^(60)Coγ ray

作　　者：廖泽云[1] 林平[1] 李玉山[1] 姜锦林[2] 

机构地区：[1]湖北民族学院医学院,湖北省恩施市445000 [2]湖北民族学院附属医院中医儿科教研室,湖北省恩施市445000

出　　处：《世界华人消化杂志》2007年第13期1541-1544,共4页World Chinese Journal of Digestology

基　　金：湖北省卫生厅科研基金项目.No.JX2C42~~

摘　　要：目的:观察长叶胡颓子多糖对小肠辐射损伤的的保护作用及其机制.方法:昆明小鼠60只随机分为:正常组、辐射组、不同剂量(100,300,500 mg/kg)胡颓子多糖给药组.采用^(60)Coγ射线一次性全身均匀照射建立小肠辐射损伤模型,相应灌胃用药2 wk后,测定小鼠肠段的肠腺存活率百分比和二胺氧化酶(DAO)含量,检测肠黏膜蛋白质和DNA含量(mg/cm),肠组织中SOD活性和MDA含量,血清中TNF、IL-4、IL-6水平.结果:不同剂量长叶胡颓子多糖灌胃均不同程度提高十二指肠、空肠和回肠段肠腺存活率(P<0.05),促进肠黏膜蛋白质、DNA合成(P<0.05或P<0.01),降低血液中TNF水平(6.89±1.33,6.74±1.24,5.87±1.13 vs 8.53±1.08;P<0.05或P<0.01)和MDA含量,提高IL-4(9.79±2.23,10.83±2.34,11.36±2.51 vs 7.40±2.08;P<0.05或P<0.01)、IL-6水平(49.36±9.65,55.26±11.32,59.68±13.65 vs 43.74±12.91;P<0.05或P<0.01)和SOD活性,且与用药剂量呈量效关系.结论:长叶胡颓子多糖通过促进肠黏膜蛋白质和DNA合成、增强抗氧化能力、调节细胞因子异常和损伤修复作用维护肠黏膜的完整性、增强屏障作用,有效减轻小肠辐射损伤.AIM： To study the protective ettect of Elaeagnus bockii Diels （EbD） polysaccharide on the small intestine of mice after whole body irradiation with ^60 γCOT ray. METHODS： Sixty Kunming mice were divided in normal group, irradiative injury group, and EbD-treated groups （100, 300, 500 mg/kg）. Irradiative injury was induced using ^60γ COT ray. EbD polysaccharide was administered by clyster once a day for two weeks. Fourteen days later, the mice were sacrificed and about 2-cm segment of duodenum, jejunum and ileum were dissected respectively for crypt survival assay. The contents of intestinal mucosal protein, DNA and diamine oxidase （DAO） were determined. The activity of superoxide dismutase （SOD）, the level of malondialdehyde （MDA） in the small intestine, the serum levels of tumor necrosis fac-tor （TNF）, interleukin-4 （IL-4） and IL-6 were also measured. RESULTS： EbD polysaccharide （100, 300, 500 mg/kg） increased crypt survival rates of duodenum, jejunum and ileum （P 〈 0.05）, promoted the synthesis of intestinal mucosal protein and DNA （P 〈 0.05 or P 〈 0.01）, enhanced the levels of IL-4 （9.79 ± 2.23, 10.83 ± 2.34, 11.36 ± 2.51 vs 7.40 ± 2.08; P 〈 0.05 or P 〈 0.01）, IL-6 （49.36 ± 9.65, 55.26 ± 11.32, 59.68 ± 13.65 vs 43.74 ± 12.91; P 〈 0.05 or P 〈 0.01） and the activity of SOD, while deceased the level of TNF （6.89 ± 1.33, 6.74 ± 1.24, 5.87 ± 1.13 vs 8.53 ± 1.08; P 〈 0.05 or P 〈 0.01） and the content of MDA. The above effect was dose dependent. CONCLUSION： EbD polysaccharide can protect the small intestine from irradiation-induced injury by promoting the synthesis of intestinal mucosal protein and DNA, modulating the abnormality of cytokines, and eliminating oxygen free radicals.

关 键 词：胡颓子多糖 小肠辐射损伤 二胺氧化酶 氧自由基 细胞因子 

分 类 号：R285.5[医药卫生—中药学]