新型神经支架材料的构建及其与骨髓基质干细胞复合的实验研究  

Development of a new type nerve tissue engineering scaffolds and its combination with bone marrow stromal cells

作　　者：孟浩[1] 罗卓荆[1] 冯林杰[1] 王亮[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,710032

出　　处：《中国矫形外科杂志》2007年第12期933-934,I0003,共3页Orthopedic Journal of China

基　　金：国家863课题项目(NO:2002AA216101)

摘　　要：[目的]研制出一种可用于修复周围神经缺损的组织工程支架材料,并观察体外培养的骨髓基质干细胞在支架材料中的生长情况。[方法]以I型胶原蛋白和明胶通过冷冻干燥技术制备胶原蛋白支架材料。扫描电镜观察内部结构的排列规律及走行,测量其孔径大小、孔隙率等指标。将骨髓基质干细胞复合到胶原蛋白支架材料中共培养5 d后,扫描电镜下观察其在材料内部的生长情况。[结果]构建的材料均为圆柱状,内部为孔径均匀且平行排列的微管结构,体外培养的骨髓基质干细胞成功种植在支架材料上,在材料内部生长良好。[结论]构建的支架材料具有良好的三维空间构形和生物相容性,为神经损伤的修复提供了一种新型的支架材料。[ Objective]To develop a new type nerve tissue engineering scaffolds to repair the defect of peripheral nerve and observe the growth of bone marrow stromal cells cultured in vitro on the scaffolds. [ Method ] The biomaterial was made of I-collagen and gelatin by freeze-drying method, the alignment regularities of microscopic channels and there course directions were observed under the scanning electronic microscope. The size of the micropores and the factor of porosity were also measured. The bone marrow stromal cells were seeded on the collagen scaffolds and cultured for 5 days, then the growth of bone marrow stromal cells cultured in the scaffolds was observed. [ Result] All the scaffolds were circular cylinder, the microscopic channels were ar- ranged in parallel manners, and the pore sizes of the channels were uniform. The bone marrow stromal cells were seeded in the scaffolds successfully. [ Conclusion] The developed nerve tissue engineering scaffolds have good structure and biocompatibility, which can be used in the repair of the nerve injuries.

关 键 词：组织工程 胶原 明胶 骨髓基质干细胞 

分 类 号：R318.08[医药卫生—生物医学工程]