高压氧对大鼠肢体缺血再灌注致心肺损伤的作用  

Effects of hyperbaric oxygen on cardiac and pulmonary impairment caused by rat hind limb ischemia/reperfusion

作　　者：原标[1] 高春锦[2] 王国忠[2] 陈大志[3] 

机构地区：[1]北京朝阳医院血管外科,北京100020 [2]北京朝阳医院高压氧科,北京100020 [3]北京朝阳医院普外科,北京100020

出　　处：《中华物理医学与康复杂志》2007年第6期379-381,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：北京市首都医学发展基金(2002-1027)

摘　　要：目的探讨高压氧(HBO)对大鼠肢体缺血再灌注(I/R)致心、肺损伤的作用及其机制。方法复制I 2 h/R 1 h模型,分为假手术组、I/R模型组、HBO治疗组。将心、肺组织行电镜病理检查;记录血清血小板膜糖蛋白CD31、CD61平均荧光强度和CD62p的阳性率。结果与I/R模型组比较。HBO治疗组心、肺组织病理改变减轻。与假手术组比较,I/R模型组CD31、CD61及CD62p均有升高,其中CD31和CD62p升高显著(P<0.05);与I/R模型组比较,HBO治疗组CD31、CD61及CD62p均有下降趋势,其中CD31和CD62p下降尤为显著(P<0.05)。结论早期行HBO治疗可显著减少急性下肢I/R大鼠模型血小板膜糖蛋白CD31和CD62p的表达,可能有助于减轻下肢I/R继发的心、肺损伤。Objective To explore the potential protective effect of HBO on cardiac and pulmonary impairment associated with hind limb isehemia/reperfusion in rat model and the underlying mechanisms. Methods Thirty Adult male Sprague-Dawley rats weighing 220 - 250 g were used in the study. Bilateral hind limb isehemia was in- duced by applying an atraumatie clamp at the end of abdominal aorta adjacent to junction site of bilateral femoral arteries. At the end of the ischemic period, reperfusion of the limbs was achieved hy releasing the clamp, The experimental animals were randomly divided into three groups： a sham surgical group, a isehemia/reperfusion （I/R） group and a hyperbaric oxygen （HBO） group. The ultrastruetural histopathologieal changes in heart and lungs were studied. The mean immunofluoreseenee intensity of CD31, CD61 or positive percentage of CD62p were measured by the flow eytometry. Results In I/R group, extensive ultrastruetural histopathologieal impairment of heart and lung were observed. The ultrastruetural histopathologieal changes in HBO group were significantly alleviated. Compared with sham surgical group, the expression of platelet membrane glyeoprotein CD31 and CD62p in the I/R group was much higher （P 〈0.05）. The expression of CD31,CD62p in HBO group was much lower than that in I/R group（P 〈 0.05）. Conclusion HBO can significantly alleviate the pathological changes in heart and lungs caused by hind limb ischemia/reperfusion in the rat model.

关 键 词：高压氧 缺血再灌注 激光多普勒 血小板膜糖蛋白 

分 类 号：R459.6[医药卫生—治疗学]