人参炔醇对氧应激诱导大鼠海马神经细胞凋亡的影响  

Effect of Panaxynol on Apoptosis of Rat Hippocampus Neurons Induced by H_2O_2

作　　者：王泽剑[1] 聂宝明[1] 殷明[2] 陆阳[1] 

机构地区：[1]上海交通大学医学院,上海200025 [2]上海交通大学药学院,上海200034

出　　处：《中国药学杂志》2007年第13期987-990,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30371731);教育部高等学校博士点基金资助项目(20050266003);上海第二医科大学博士点基金资助项目(BXJ0503)

摘　　要：目的探讨人参炔醇对原代大鼠海马神经细胞氧应激的保护作用。方法通过MTT和LDH法、AO/EB双染色法检测人参炔醇对神经元氧应激诱导凋亡的影响;通过使用信号转导阻断剂研究人参炔醇抗凋亡涉及的信号转导通路。结果人参炔醇对H2O2造成的海马神经细胞损伤有浓度依赖性的保护作用,可促进细胞存活,减少细胞的凋亡率;人参炔醇的神经保护作用可被RpcAMPs和wortmannin阻断,可被genistein和H7部分阻断,而PD98059对人参炔醇的神经保护作用无显著影响。结论人参炔醇保护神经元对抗氧应激,主要与细胞内cAMP及PI3K系统活性增高有关。OBJECTIVE To investigate the protective effect of panaxynol on primary cultured hippocampus neurons. METHODS MTT reduction and LDH release were used to assay cell viability. AO/EB double staining was used to evaluate the apoptotic neurons, and the signal pathways involved were screened by the treatment of kinases blockers. RESULTS Panaxynol alleviated the H2O2-induced toxicity to hippocampus neurons concemtration-dependently and reduced the apoptotic rate. The protective effect of panaxynol was inhibited by RpcAMPs and wortmannin completely, and inhibited by genistein and H7 partly, while PD98059 had no influence on it, CONCLUSION Panaxynol possessed protective effect on hippocampus neurons which mainly involved the activation of adenylyl cyclase and PI3K pathways.

关 键 词：人参炔醇 海马神经元 过氧化氢 信号转导 

分 类 号：R965[医药卫生—药理学]