聚合酶链式反应与DNA探针检测临床标本中结核分支杆菌的评价  

Evaluation of Detection of M. Tuberculosis in Clinical Specimens by PCR Combining Dig-DNA Probe

作　　者：李小英[1] 张捷[1] 寇丽筠[1] 邓蕾[1] 

机构地区：[1]北京医科大学第三医院,100083

出　　处：《中国防痨杂志》1997年第1期33-35,共3页Chinese Journal of Antituberculosis

摘　　要：本文应用ＰＣＲ联合ＤＮＡ探针快速检测临床标本中结核杆菌ＤＮＡ。对临床３０７例标本同时进行ＰＣＲ和Ｓｏｕｅｈｅｒｎ杂交。其中临床确诊为结核病的１０９例，二者的阳性率分别为４９．６％、６０．６％。未确诊结核病的１９８例，二者均为阳性２例、单纯ＰＣＨ阳性３例。结果表明ＤＮＡ探针检测的灵敏性和特异性高于ＰＣＲ。二者联合使用，有助于对疑难电泳图谱的分析和判断，降低假阴性和假阳性率。Tubercle bacillus (TB) DNA was detected by using PCR combining Southern blot hybridization of TB DNA probes labeled with digoxin in 307 cases, among which 109 cases with clinically confirmed tuberculosis. The positive rates of PCR alone and PCR combining Southern blot were 49. 6% and 60. 6% respectively. In the remaining 198 cases without clini- cally confirmed tuberculosis, 2 cases were both PCR hybridization positive and 3 cases were PCR positive. Conclusions: PCR in combination with Southern hybridization is more sensitive and specific than PCR alone and helpful to the analysis of some non-typical electrophoresis patterns.

关 键 词：结核杆菌 DNA探针 聚合酶链式反应 

分 类 号：R520.4[医药卫生—内科学] R446.5[医药卫生—临床医学]