HPLC法同时测定氯霉素醋酸泼尼松龙滴眼液中两组分含量  

Concurrent Determination of Chloramphenicol and Prednisolone Acetate in Chloramphenicol and Prednisolone Acetate Eye Drops by HPLC

作　　者：杨宏昕[1] 

机构地区：[1]内蒙古自治区医院,呼和浩特010017

出　　处：《中国药事》2007年第6期410-411,414,共3页Chinese Pharmaceutical Affairs

摘　　要：建立氯霉素醋酸泼尼松龙滴眼液的含量测定方法。采用高效液相色谱法：ALLTECH C18色谱柱，流动相为甲醇-水（70：30），流速为0．7mL·min^-1，检测波长为242nm，柱温为25℃。氯霉素和醋酸泼尼松龙分别在11．88-237．6μg·mL^-1（r=0．9994）和4．752-95．04μg·mL^-1（r=0．9998）范围内峰面积与其浓度线性关系良好，平均回收率依次分别为100．0％（RSD=1．1％）、100．9％（RSD=0．8％）。本方法可同时测定两种成分含量，操作简便，结果准确。To establish a HPLC method for concurrent determination of chloramphenicol and prednisolone acetate of chloramphenicol and prednisolone acetate eye drops. The HPLC system consisted ALLTECH C18 column , methanol-water （70 ： 30） as mobile phase with detection wave length at 242 nm, flow rate was 0. 7mL · min^-1, temperature of column was 25 ℃. The linear range of chloramphenicol and prednisolone acetate were within 11.88 -237. 6 μg · mL^-1 （r=0. 9994） and 4. 752 -95.04μg · mL^-1 （r=0.9994）, the average recovery was 100.0% （RSD=1. 1%）, 100.9% （RSD=0.8%）. The established method is suitable for the concurrent determination of the two components. The method is convenient and accurate.

关 键 词：高效液相色谱法 氯霉素 醋酸泼尼松龙 

分 类 号：R927.2[医药卫生—药学]