高效液相色谱法测定果蔬中残留的噻苯咪唑、邻苯基苯酚及联苯  

HPLC simultaneous determination of thiabendazole,o-phenylphenol and diphenyl residues in fruits and vegetables

作　　者：沈静[1] 李明 艾尔肯.依不拉音 陈坚[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830054 [2]新疆产品质量监督检验研究院,乌鲁木齐830002

出　　处：《药物分析杂志》2007年第6期921-924,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(20565004);新疆自治区科技攻关及重点科技项目(200532111)

摘　　要：目的:建立同时测定果蔬中残留的噻苯咪唑(TBZ)、邻苯基苯酚(OPP)及联苯(DP)的 HPLC 法,并对乌鲁木齐地区部分高档水果中保鲜剂的残留状况进行初步评估。方法:在碱性条件下,用乙醚提取果蔬中 TBZ、OPP 及 DP 3种保鲜剂,以甲醇-乙腈-5 mmol·L^(-1)十二烷基磺酸钠溶液(磷酸调 pH:4.5)(55:10:35)为流动相,流速1.0 mL·min^(-1),荧光检测(λ_(ex)=285nm,λ_(em)=320 nm),进样量10μL。结果:TBZ、OPP 及 DP 的线性范围分别为0.01～8,0.007～10,0.01～20μg·mL^(-1);相关系数均大于0.999;最低检测限分别为0.01,0.007,0.01 μg·mL^(-1);平均回收率为71.4%～93.2%;RSD 为0.6%～6.5%。结论:本实验建立的 HPLC-荧光检测,方法准确可靠,适合于果蔬中噻苯咪唑、邻苯基苯酚及联苯残留的测定。Objective：To establish an HPLC method for the determination of three fungicide residues [ thiabendazole（TBZ） ,o- phenylphenol（OPP）, diphenyl（DP） ] in fruit and vegetable and initially assess the amount of residues in fruit in Urumqi markets. Methods：The residues were extracted under basic conditions with ethyl ether. These compounds were determined by liquid chromatography -fluorescence detection （hex = 285 nm, hem = 320 nm）. A mobile phase consisting of methanol - acetonitrile - 5 mmol · L^ - 1 SDS （ adjusted pH to 4. 5 with phosphoric acid） （55： 10： 35） gave the best results. The flow rate was set at 1.0 mL · min^- 1 Result：The correlation coefficients were above 0. 999. The linear range of TBZ, OPP, DP were 0. 01 -8,0. 007 - 10,0. 01 -20 μg · mL^-1 and limits 0. 01,0. 007,0. 01μ g · mL^-1 ,respectively. The average recoveries from spiked fruit and vegetable were from 71.4% to 93, 2% and RSD were from 0. 6% to 6. 5%. Conclusion：This method is accurate,reliable and suitable for the determination of TBZ, OPP and DP residues in fruit and vegetable.

关 键 词：噻苯咪唑(TBZ) 邻苯基苯酚(OPP) 联苯(DP) 高效液相色谱法 食品保鲜剂残留分析 

分 类 号：R917[医药卫生—药物分析学]