鸡大肠杆菌(O_(78))1型菌毛单克隆抗体研制及对分离菌株的检测  

Preparation of Monoclonal Antibodies Specific to Type 1 Pili of Avian Escherichia coli and Their Detection to Isolation Strains

作　　者：戴鼎震[1] 陈钟鸣[1] 王晓丽[2] 张志成[1] 

机构地区：[1]金陵科技学院动物科学与技术学院,江苏南京210038 [2]江苏省农业科学院兽医研究所,江苏南京210014

出　　处：《金陵科技学院学报》2007年第2期92-96,共5页Journal of Jinling Institute of Technology

基　　金：国家自然科学基金资助项目(30070569);江苏省自然科学基金资助项目(BK2005107);江苏省高校自然科学基金基础研究资助项目(07KJD230062)

摘　　要：将菌毛化的鸡大肠杆菌国内分离株GL7（O78）灭活后作为免疫原,经淋巴细胞杂交瘤技术,用间接酶联免疫吸附试验（ELISA）筛选,获得3E1、3H1、4A9和5G4共4株能稳定分泌1型菌毛抗体的杂交瘤细胞,它们均为IgG。免疫印迹试验结果表明：4株单抗均能同鸡大肠杆菌1型菌毛反应,识别分子质量为17-17.5 ku的菌毛蛋白。同时对鸡大肠杆菌表达1型菌毛的分离株进行检测,结果表明同一血清型或不同血清型间的鸡病原性大肠杆菌1型菌毛之间存在差异,O血清型不能完全体现菌株间的遗传相关性。Avian Escherichia coli isolated strain GL7 （O78）, was inactivated as immunogen to prepare specific monoclonal antibody. 4 strains of monoclonal antibody hybridoma cells were obtained by indirectly enzyme-linked immunosorbent assay （ELISA）. They are all IgG, all the 4 strains were specific to the type 1 pill through Western-blotting, and recognized the protein of fimbriae which MW about 17 - 17.5 ku. There antibodies were used as probe to detect the type 1 from Escherichia coli isolated strain from chicken, ware in the same serotype or not, the result showed the there ware exist differences whether there and O serotype can＇t fully embody the hereditary relativity of different strains.

关 键 词：鸡大肠杆菌 1型菌毛 单克隆抗体 检测 

分 类 号：Q939.121[生物学—微生物学]