华山松木蠹象性信息素初步研究  

A Preliminary Study on the Sex Pheromone of Armand Pine Bark-weevil(Pissodes punctatus)

作　　者：李永和[1] 陈敏[2] 叶辉[1] 

机构地区：[1]云南大学,云南昆明6500911 [2]西南林学院,云南昆明650224

出　　处：《西北林学院学报》2007年第4期115-118,共4页Journal of Northwest Forestry University

基　　金：云南省"十五"科技攻关项目(2003NG12)

摘　　要：为利用华山松木蠹象性信息素防治华山松木蠹象提供理论依据,对华山松木蠹象性信息素进行了初步研究,结果如下:(1)通过室内观察,确定华山松木蠹象信息素释放的高峰期在19:00～20:00,华山松木蠹象信息素活性粗提物的制取应在这一时段进行;(2)华山松木蠹象雌成虫性信息素粗提物的生物测定结果表明,用甲醇和乙醇作为溶剂浸提雌成虫性信息素腺体,所得粗提物对雄成虫均显示引诱活性,甲醇浸提液显示出较强的活性;(3)采用GC/MS方法对性信息素粗提物的成分进行分析,甲醇和乙醇作为提取溶剂得到的性信息素粗提物均分离出12种化学物质。用甲醇作为提取液得到的粗提物鉴定出6种化合物,为十一烷、十二烷、十六碳烯酸甲酯、十六烷酸甲酯、十八碳烯酸甲酯和十八烷酸甲酯;用乙醇作为提取液得到的粗提物鉴定出化合物5种,为十六烷酸甲酯、邻苯二甲酸甲酯、十八碳烯酸甲酯、十八碳二烯酸甲酯、十八碳酸甲酯。To provide the theoretical basis for the management of armand pine bark-weevil, the sex pheromone of armand pine bark-weevil was studied preliminarily . The results were as follows ： （ 1 ） The peak hours that armand pine bark-weevils secreted their sex pheromone were at 19：00 - 20：00 by observing their mating behavior in the laboratory. So sex pheromone must be extracted in this period ; （2） Bioassays indicated that the primary extract of sex pheromone from female armand pine bark-weevils pheromone gland both using the methanol and ethanol as solvents showed certain attraction for male armand pine bark-weevils, and the first one showed a stronger attraction ; （3） GC/MS was used to analyze the constituents of the primary extract of sex pheromone. 12 compounds were found in methanol extract, and 6 were identified： undecane, dodecane ,methyl （z）-9-hexadecenate, methyl hexadecanate, methyl 9-octadecenate, methyl octadecanate. 12 compounds were also found in methanol extract, and 5 were identified ： methyl hexadecanate, methyl phthalate, methyl （ z, z） -9,12-octadecadienate, methyl 9-octadecenate, methyl octadecanate.

关 键 词：华山松木蠹象 性信息素 提取方法 生物测定 成分分析 

分 类 号：S763.306.9[农业科学—森林保护学]