雌二醇诱导小鼠腹腔巨噬细胞程序性死亡  

ESTRADIOL INDUCES APOPTOSIS IN MOUSE PERITONEAL MA CROPHAGES

作　　者：张修武[1] 牛喜林[1] 郭兆贵[1] 

机构地区：[1]湖南医科大学分子药理研究室

出　　处：《免疫学杂志》1997年第1期14-16,共3页Immunological Journal

摘　　要：研究雌二醇和它莫西芬对培养的小鼠腹腔巨噬细胞的影响，发现雌二醇能够诱导巨噬细胞产生典型的细胞程序性死亡的形态学改变和特征性的ＤＮＡ“梯形”结构，而它莫西芬能够抑制雌二醇诱导细胞程序性死亡的作用。用１μｍｏｌ／Ｌ，１００ｎｍｏｌ／Ｌ，１０ｎｍｏｌ／Ｌ的１７，β－雌二醇处理巨噬细胞１２ｈ后出现典型的ＤＮＡ“梯形”结构，呈剂量依赖方式。１０μｍｏｌ／Ｌ的它莫西芬能够有效抑制１μｍｏｌ／Ｌ的１７，β－雌二醇诱导的巨噬细胞程序性死亡。Ｓｔａｕｒｏｓｐｏｒｉｎｅ和放线菌酮能够抑制雌二醇诱导巨噬细胞产生的典型的ＤＮＡ“梯形”结构的形成。To investigate whether estradiol might induce apoptosis in mouse peritoneal macrophages,the mouse peritoneal macrophages were isolated and incubated in culture medium containing 17 beta estradiol,17 beta estradiol and tamoxifen,or staurosporine,or cycloheximide,and DNA fragmentation was visualized by agarose gel electrophoresis.Estradiol elicited typical apoptosis morphological changes and DNA fragmentation in dose and time dependent manner.The results show that 1 μmol/L,100 nmol/L,10nmol/L estradiol incubated with macrophages for 12h could induce a typical DNA ladder in dose dependent manner;10μmol/L tamoxifen could inhibit apoptosis induced by 1 μmol/L estradiol in macrophages;20nmol/L staurosporine and 1 μg/ml cycloheximide could inhibit the DNA fragmentation.In conclusion,estradiol was able to induce apoptosis in mouse peritoneal macrophages,and tamoxifen could inhibit it.Protein kinase C and protein synthesis were involved in this event.

关 键 词：雌二醇 它莫西芬 巨噬细胞 程序性死亡 

分 类 号：R392.12[医药卫生—免疫学] Q73[医药卫生—基础医学]