狂犬疫苗与白细胞介素2联合免疫小鼠的实验研究  

INTERLEUKIN 2 AS AN ADJUVENT TO INCREASE THE IMMUNE RESPONSE TO INACTIVATED RABIES VIRUS VACCINE

作　　者：刘劲松[1,2,3] 陆虹 李燕[1,2,3] 邹全明[1,2,3] 周为民[1,2,3] 崔景辉[1,2,3] 宋绍勇[1,2,3] 伊纯德 张守峰[1,2,3] 

机构地区：[1]大连医学高等专科学校病原教研室 [2]中国预防医学科学院病毒学研究所 [3]第三军医大学临床微生物学教研室

出　　处：《免疫学杂志》1997年第1期23-25,共3页Immunological Journal

摘　　要：组织培养制备的狂犬病毒灭活疫苗与重组白细胞介素２同时免疫ＢＡＬＢ／ｃ鼠和Ｓｗｉｓｓ鼠，检测不同时间的鼠血清中抗病毒ＩｇＧ抗体和中和抗体效价。结果显示：在白细胞介素２的作用下，抗体产生的时间提前，抗体效价提高（Ｐ＜０．０１），ＢＡＬＢ／ｃ鼠和Ｓｗｉｓｓ鼠比较，前者的抗体效价更高（Ｐ＜０．０１）。狂犬疫苗与白细胞介素２联合免疫后ＮＫ细胞杀伤活性显著提高，达５１．３７％（Ｐ＜０．０１），特异性细胞毒性Ｔ细胞（ＣＴＬ）活性的二次反应显著加强（Ｐ＜０．０１）。In determining whether the interleukin2(IL 2) could be an adjuvent to increase the immune response of mouse to inactivated radies virus vaccine.BALB/c mice and Swiss mice were divided equally into two groups respectively.They were immunized by the inactivated rabies virus vaccine with IL 2 or without IL 2,and detected for anti virus IgG antibody and virus neutralizing antibody after vaccination.The results showed that the anti virus IgG antibodies and virus neutralizing antibodies had appeared 5 days earlier for the group with IL 2 than the one without IL 2.The antibody titer had raised much higher for the group with IL 2 than another one.Similarly,as an adjuvent,the IL 2 was able to increase the cellular immune response to the vaccine.CBA/J mice were immunized some vaccine and IL 2,the activity of nature killer cells(NK)and the activity of specific cytotoxic T lymphocytes(CTL)reached a high level,they were 51.3±6.7% and 29.1±3.0%,respectively.

关 键 词：狂犬疫苗 白细胞介素2 细胞免疫 

分 类 号：R392.7[医药卫生—免疫学] R512.990.3[医药卫生—基础医学]